TAILIEUCHUNG - Báo cáo khoa học: Evidence for the presence of ferritin in plant mitochondria

In this work, evidence for the presence of ferritins in plant mitochondria is supplied. Mitochondria were isolated from etiolated pea stems andArabidopsis thalianacell cultures. The proteinswere separatedbySDS/, with an apparent molecular mass of approximately 25–26 kDa (corresponding to that of ferritin), was cross-reactedwith an antibody raised against pea seed ferritin. The mitochondrial ferritin from pea stems was also purified by immunopre-cipitation. | Eur. J. Biochem. 271 3657-3664 2004 FEBS 2004 doi Evidence for the presence of ferritin in plant mitochondria Marco Zancani1 Carlo Peresson1 Antonino Biroccio2. Gioraio Federici2. Andrea Urbani3 Irene Muraia4 Carlo Soave4 Fulvio Micali5 Angelo Vianello1 and Francesco Macri1 1 Dipartimento di Biologia ed Economia Agro-Industriale Sezione di Biologia Vegetale Universita di Udine Italy 2Laboratorio di Biochimica Clinica Ospedale Pediatrico del Bambino Gesu - IRCCS Roma Vatican State 3 Centro Studi sull Invecchiamento Facolta di Medicina e Chirurgia Dipartimento di Scienze Biomediche Universiia G. D Annunzio Chieti Italy 4Dipartimento di Biologia Sezione di Fisiologia e Biochimica delle Piante Universita di Milano Italy 5Dipartimento di Biochimica Biofisica e Chimica delle Macromolecole Universita di Trieste Italy In this work evidence for the presence of ferritins in plant mitochondria is supplied. Mitochondria were isolated from etiolated pea stems and Arabidopsis thaliana cell cultures. The proteins were separated by SDS PAGE. A protein with an apparent molecular mass of approximately 25-26 kDa corresponding to that of ferritin was cross-reacted with an antibody raised against pea seed ferritin. The mitochondrial ferritin from pea stems was also purified by immunoprecipitation. The purified protein was analyzed by MALDI-TOF mass spectrometry and the results of both mass finger print and peptide fragmentation by post source decay assign the polypeptide sequence to the pea ferritin P . The mitochondrial localization of ferritin was also confirmed by immunocytochemistry experiments on isolated mitochondria and cross-sections of pea stem cells. The possible role of ferritin in oxidative stress of plant mitochondria is discussed. Keywords ferritin iron mitochondria Arabidopsis thaliana Pisum sativum. Iron is an essential element for all living organisms 1 . In green plants its importance mainly derives from the presence at the

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