TAILIEUCHUNG - Báo cáo khoa học: The splicing factor ASF/SF2 is associated with TIA-1-related/ TIA-1-containing ribonucleoproteic complexes and contributes to post-transcriptional repression of gene expression

TIA-1-related (TIAR) protein is a shuttling RNA-binding protein impli-cated in several steps of RNA metabolism. In the nucleus, TIAR contrib-utes to alternative splicing events, whereas, in the cytoplasm, it acts as a translational repressor on specific transcripts such as adenine and uridine-rich element-containing mRNAs. | The splicing factor ASF SF2 is associated with TIA-1-related TIA-1-containing ribonucleoproteic complexes and contributes to post-transcriptional repression of gene expression Nathalie Delestienne1 Corinne Wauquier1 Romuald Soin1 Jean-Francois Dierick2 Cyril Gueydan1 1 and Veronique Kruys1 1 1 Laboratoire de Biologie Moleculaire du Gene Faculte des Sciences Universite Libre de Bruxelles Gosselies Belgium 2 Biovallee Proteomics Unit Charleroi Belgium Keywords AU-rich elements hnRNP heterogenous nuclear ribonucleoprotein ribonucleoprotein complexes RNA metabolism RNA-binding proteins stress granules Correspondence V. Kruys Laboratoire de Biologie Moleculaire du Gene Institut de Biologie et de Medecine Moleculaires Universite Libre de Bruxelles 12 rue des Profs. Jeener et Brachet 6041 Gosselies Belgium Fax 32 2 6509800 Tel 32 2 6509804 E-mail vkruys@ Present address GSK Biologicals Wavre Belgium Fhese authors contributed equally to this work Received 10 January 2010 revised 10 March 2010 accepted 25 March 2010 doi TIA-1-related TIAR protein is a shuttling RNA-binding protein implicated in several steps of RNA metabolism. In the nucleus TIAR contributes to alternative splicing events whereas in the cytoplasm it acts as a translational repressor on specific transcripts such as adenine and uridine-rich element-containing mRNAs. In addition TIAR is involved in the general translational arrest observed in cells exposed to environmental stress. This activity is encountered by the ability of TIAR to assemble abortive pre-initiation complexes coalescing into cytoplasmic granules called stress granules. To elucidate these mechanisms of translational repression we characterized TIAR-containing complexes by tandem affinity purification followed by MS. Amongst the identified proteins we found the splicing factor ASF SF2 which is also present in TIA-1 protein complexes. We show that although mostly confined in the nuclei of normal cells .

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