TAILIEUCHUNG - Báo cáo khoa học: Protein–protein interactions and selection: yeast-based approaches that exploit guanine nucleotide-binding protein signaling

For elucidating protein–protein interactions, many methodologies have been developed during the past two decades. For investigation of interac-tions inside cells under physiological conditions, yeast is an attractive organism with which to quickly screen for hopeful candidates using versa-tile genetic technologies, and various types of approaches are now avail-able. | MINIREVIEW Protein-protein interactions and selection yeast-based approaches that exploit guanine nucleotide-binding protein signaling Jun Ishii1 Nobuo Fukuda2 Tsutomu Tanaka1 Chiaki Ogino2 and Akihiko Kondo2 1 Organization of Advanced Science and Technology Kobe University Japan 2 Department of ChemicalScience and Engineering Graduate Schoolof Engineering Kobe University Japan Keywords guanine nucleotide-binding protein protein-protein interaction screening signaling yeast yeast two-hybrid Correspondence A. Kondo Department of Chemical Science and Engineering Graduate School of Engineering Kobe University 1-1 Rokkodaicho Nada-ku Kobe 657-8501 Japan Fax 81 78 803 6196 Tel 81 78 803 6196 E-mail akondo@ These authors contributed equally to this work Received 29 October 2009 revised 5 February 2010 accepted 24 February 2010 doi For elucidating protein-protein interactions many methodologies have been developed during the past two decades. For investigation of interactions inside cells under physiological conditions yeast is an attractive organism with which to quickly screen for hopeful candidates using versatile genetic technologies and various types of approaches are now available. Among them a variety of unique systems using the guanine nucleotide-binding protein G-protein signaling pathway in yeast have been established to investigate the interactions of proteins for biological study and pharmaceutical research. G-proteins involved in various cellular processes are mainly divided into two groups small monomeric G-proteins and heterotrimeric G-proteins. In this minireview we summarize the basic principles and applications of yeast-based screening systems using these two types of G-protein which are typically used for elucidating biological protein interactions but are differentiated from traditional yeast two-hybrid systems. Introduction Protein-protein interactions have fundamental roles in a variety of biological .

• Báo cáo khoa học
• Scientific reports
• scientific research
• molecular Biology
• biological activities
• gastric cancer
• Crystal structure
• collection of scientific reports
• industry reports philosophy of history reports
• reports in literature
• a scientific b shirt
• presentation of scientific reports
• reports on literature
• philosophy statement
• documents
• studies English language and English language communication and English language commercial
• progress reports at scientific collections
• agronomic reports
• reports in physics
• biology reports
• thematic biological
• induced geometry
• mathematical topics
• quantum mechanics
• physics
• reporting mathematics
• physics reports
• livestock reports
• physical reports
• research physicist
• specializing in physics
• mathematical statements
• mathematical equations
• English pronunciation
• presentation report
• agricultural reports
• technical crops
• bio technology
• English reports
• presentation reports
• mathematical knowledge
• reports of mathematics
• represented
• Wilf on packing distinct
• Tur´n density
• mathematical works
• patterns in a permutation
• statements and documents scientific reports
• reports agronomy
• biotechnology
• plants Vietnam
• specialized biological
• microbiological methods
• presenting the report
• anti bacterial properties of shrimp
• energy exchange
• engineering chicken
• Path counting
• random matrix theory
• Even circuits
• prescribed clockwise parity
• Correspondence between
• algorithmic characterizations
• Finding Induced Acyclic
• Random Digraphs
• Nonexistence results
• Hadamard like matrices
• Voltage Graphs
• Group Presentations
• Cages mathematical knowledge
• Dominance Order
• Graphical Partitions
• On the functions
• values in [α(G
• Box With Translates
• Two Given Rectangular Brick
• On the q analogue
• the sum of cubes