TAILIEUCHUNG - Báo cáo khoa học: Identification and characterization of an R-Smad ortholog (SmSmad1B) from Schistosoma mansoni

Smad proteins are the cellular mediators of the transforming growth fac-tor-b superfamily signals. Herein, we describe the isolation of a fourth Smad gene from the helminthSchistosoma mansoni, a receptor-regulated Smad (R-Smad) gene termedSmSmad1B. The SmSmad1B protein is com-posed of 380 amino acids, and contains conserved MH1 and MH2 domains separated by a short 42 amino acid linker region. | ỊFEBS Journal Identification and characterization of an R-Smad ortholog SmSmadlB from Schistosoma mansoni Joelle M. Carlo1 Ahmed Osman1 2 Edward G. Niles1 Wenjie Wu2 Marcelo R. Fantappie2 Francisco M. B. Oliveira2 and Philip T. LoVerde1 2 1 Department of Microbiology and Immunology Schoolof Medicine and BiomedicalSciences State University of New York NY USA 2 Southwest Foundation for BiomedicalResearch San Antonio TX USA Keywords bone morphogenic protein Schistosoma mansoni Smad transforming growth factor-b Correspondence P. T. LoVerde South-west Foundation for BiomedicalResearch PO Box 7620 NW Loop 410 San Antonio TX 78227-5301 USA Fax 1 210 670 3322 Tel 1 216 258 5892 E-mail ploverde@ Database The nucleotide sequence described here is available in the GenBank database under the accession number AY666164 These authors contributed equally to this work Received 14 February 2007 revised 5 June 2007 accepted 11 June 2007 doi Smad proteins are the cellular mediators of the transforming growth factor-b superfamily signals. Herein we describe the isolation of a fourth Smad gene from the helminth Schistosoma mansoni a receptor-regulated Smad R-Smad gene termed SmSmadlB. The SmSmad1B protein is composed of 380 amino acids and contains conserved MH1 and MH2 domains separated by a short 42 amino acid linker region. The SmSmadlB gene kb is composed of five exons separated by four introns. On the basis of phylogenetic analysis SmSmad1B demonstrates homology to Smad proteins involved in the bone morphogenetic protein pathway. Sm-Smad1B transcript is expressed in all stages of schistosome development and exhibits the highest expression level in the cercariae stage. By immunolocalization experiments the SmSmad1B protein was detected in the cells of the parenchyma of adult schistosomes as well as in female reproductive tissues. Yeast two-hybrid experiments revealed an interaction between Sm-Smad1B and the common Smad SmSmad4. As .

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