TAILIEUCHUNG - Báo cáo khoa hoc : Effect of the N-terminal sequence on the binding affinity of transthyretin for human retinol-binding protein

During vertebrate evolution, the N-terminal region of transthyretin (TTR) subunit has undergone a change in both length and hydropathy. This was previously shown to change the binding affinity for thyroid hormones (THs). However, it was not known whether this change affects other functions of TTR. In the present study, the effect of these changes on the binding of TTR to retinol-binding protein (RBP) was determined. | IFEBS Journal Effect of the N-terminal sequence on the binding affinity of transthyretin for human retinol-binding protein Ladda Leelawatwattana1 Verayuth Praphanphoj2 and Porntip Prapunpoj1 1 Department of Biochemistry Faculty of Science Prince of Songkla University Hat Yai Songkhla Thailand 2 The Center of MedicalGenetics RajanukulInstitute Din-Daeng District Bangkok Thailand Keywords binding affinity evolution retinol-binding protein structure and function transthyretin Correspondence P. Prapunpoj Department of Biochemistry Faculty of Science Prince of Songkla University Hat Yai Songkhla 90110 Thailand Fax 66 74 446656 Tel 66 74 288275 E-mail Received 5 April 2011 revised 9 July 2011 accepted 13 July 2011 doi During vertebrate evolution the N-terminal region of transthyretin TTR subunit has undergone a change in both length and hydropathy. This was previously shown to change the binding affinity for thyroid hormones THs . However it was not known whether this change affects other functions of TTR. In the present study the effect of these changes on the binding of TTR to retinol-binding protein RBP was determined. Two wild-type TTRs from human and Crocodylus porosus and three chimeric TTRs including a human chimeric TTR in which its N-terminal sequence was changed to that of C. porosus TTR croc huTTR and two C. porosus chimeric TTRs hu crocTTR in which its N-terminal sequence was changed to that of human TTR and xeno crocTTR in which its N-terminal sequence was changed to that of Xenopus laevis TTR were analyzed for their binding to human RBP by native-PAGE followed by immunoblotting and a chemilluminescence assay. The Kd of human TTR was M and was similar to that reported for the second binding site whereas that of crocodile TTR was IM. The binding affinities increased in croc huTTR Kd im and xeno crocTTR Kd im in which their N-termini were longer and more hydrophobic

• Báo cáo khoa học
• Scientific reports
• medical analysis
• environmental medicine
• biochemical studies
• medical knowledge
• medical research
• collection of scientific reports
• industry reports philosophy of history reports
• reports in literature
• a scientific b shirt
• presentation of scientific reports
• reports on literature
• philosophy statement
• documents
• studies English language and English language communication and English language commercial
• progress reports at scientific collections
• agronomic reports
• reports in physics
• biology reports
• thematic biological
• induced geometry
• mathematical topics
• quantum mechanics
• physics
• reporting mathematics
• physics reports
• livestock reports
• physical reports
• research physicist
• specializing in physics
• mathematical statements
• mathematical equations
• English pronunciation
• presentation report
• agricultural reports
• technical crops
• bio technology
• English reports
• scientific research
• presentation reports
• mathematical knowledge
• reports of mathematics
• represented
• Wilf on packing distinct
• Tur´n density
• mathematical works
• patterns in a permutation
• statements and documents scientific reports
• reports agronomy
• biotechnology
• plants Vietnam
• specialized biological
• microbiological methods
• presenting the report
• anti bacterial properties of shrimp
• energy exchange
• engineering chicken
• Path counting
• random matrix theory
• Even circuits
• prescribed clockwise parity
• Correspondence between
• algorithmic characterizations
• Finding Induced Acyclic
• Random Digraphs
• Nonexistence results
• Hadamard like matrices
• Voltage Graphs
• Group Presentations
• Cages mathematical knowledge
• Dominance Order
• Graphical Partitions
• On the functions
• values in [α(G
• Box With Translates
• Two Given Rectangular Brick
• On the q analogue
• the sum of cubes