TAILIEUCHUNG - Báo cáo khoa học: Four divergent Arabidopsis ethylene-responsive element-binding factor domains bind to a target DNA motif with a universal CG step core recognition and different flanking bases preference

TheArabidopsisethylene-responsive element-binding factor (AtERF) fam-ily of transcription factors has 120 members, all of which possess a highly conserved ERF domain. AtERF1, AtERF4, AtEBP and CBF1 are members from different phylogenetic subgroups within the family. | Four divergent Arabidopsis ethylene-responsive element-binding factor domains bind to a target DNA motif with a universal CG step core recognition and different flanking bases preference Shuo Yang1 Shichen Wang1 Xiangguo Liu1 Ying Yu1 Lin Yue3 Xiaoping Wang1 and Dongyun Hao1 2 1 Key Laboratory for Molecular Enzymology and Engineering of the Ministry of Education Jilin University Changchun China 2 Biotechnology Research Centre Jilin Academy of AgriculturalSciences JAAS Changchun China 3 Schoolof PhysicalEducation Northeast NormalUniversity Changchun China Keywords CG step DRE motif ERF domain homology universal binding characteristic Correspondence D. Hao Biotechnology Research Centre Jilin Academy of AgriculturalSciences JAAS Changchun 130033 China Fax 86 431 87063080 Tel 86 431 87063195 E-mail dyhao@ Received 31 August 2009 revised 29 September 2009 accepted 8 October 2009 doi The Arabidopsis ethylene-responsive element-binding factor AtERF family of transcription factors has 120 members all of which possess a highly conserved ERF domain. AtERF1 AtERF4 AtEBP and CBF1 are members from different phylogenetic subgroups within the family. Electrophoretic mobility shift assay analyses revealed that the ERF domains of these four proteins were capable of binding specifically to either GCC or dehydration-responsive element DRE motifs. In vitro and in vivo binding assays of the four AtERFs with the DRE motif showed that the recognition of the CG step was indispensable in all four of the specific binding reactions implying that there may be a universal binding characteristic of various ERF domains binding to a given consensus . the DRE motif . In addition the core DNA-binding motifs preferred by the four AtERFs were identified and all of these motifs contained a conserved CG step core. Thus conserved recognition of the CG step may be the foundation of the formation of the stable complex by the ERF domain with the DRE motif which

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