TAILIEUCHUNG - Báo cáo khoa học: "Micropropagation of Araucaria columnaris Hook."

Tuyển tập các báo cáo nghiên cứu về lâm nghiệp được đăng trên tạp chí lâm nghiệp quốc tế đề tài: "Micropropagation of Araucaria columnaris Hook. | 158s Ann. Sci. For. 1989 46 suppl. 158S-16ŨS Forest Tree Physiology E. Dreyer etal. eds. Elsevier INRA Micropropagation of Araucaria columnaris Hook. L. Sehgal . Sehgal and . Khosla DYSP University of Horticulture and Forestry Solan 173230 India Introduction The genus Araucaria comprises several magnificent evergreen forest tree species including Araucaria columnaris Hook. which has a high ornamental value as well. Conventional propagation by seeds is slow and inadequate for producing large uniform progenies. Cuttings are difficult to root and they produce plagiotropic plants except when taken from apical shoots. Successful micropropagation of this species has not been reported. The aim of this study was to use explants from normally growing mature trees for in vitro propagation of A. columnaris. Materials and Methods Tips 3-5 mm of secondary and tertiary lateral branches taken from 14 yr old trees were sterilized in mercuric chloride for 1 min and cultured on Murashige and Skoog s MS medium Murashige and Skoog 1962 containing different concentrations of various growth regulators and or other chemicals. The cultures were kept at 25 c under cool white fluorescent light klux from 40 w tubes for 16 h each day. At least 20 replications were used for each treatment. Results The explants initially cultured on MS medium supplemented with kinetin 5-12 pM and naphthalene acetic acid 1-3 pM were established on the medium containing only kinetin 10 pM . The establishment of cultures was maximum 40-50 when the explants were collected in April and November whereas it was low 10-30 during other months of the year. The development of axillary buds was obtained by subculturing on a medium containing 6-benzylaminopurine BAP pM indole butyric acid IBA 1 pM and 3 sucrose. Explants taken in January prechilled for 1 mo at 5 c and cultured on MS medium containing 10 pM kinetin and 1 pM IBA Permanent address Gargi College Delhi University N. Delhi 110049 India. 159s .

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