TAILIEUCHUNG - Báo cáo khoa học: X-ray crystallographic and enzymatic analyses of shikimate dehydrogenase from Staphylococcus epidermidis

The 4-aminobutyrate type A receptor-associated protein (GABARAP) is a versatile adaptor protein that plays an important role in intracellular vesi-cle trafficking, particularly in neuronal cells. We have investigated the structural determinants underlying the interaction of GABARAP with cal-reticulin using spectroscopic and crystallographic techniques. | ỊFEBS Journal X-ray crystallographic and enzymatic analyses of shikimate dehydrogenase from Staphylococcus epidermidis Implications for substrate binding and conformational change Cong Han1 z Tiancen Hu2 z Dalei Wu2 Su Qu1 Jiahai Zhou3 Jianping Ding4 Xu Shen2 Di Qu1 and Hualiang Jiang2 1 Institutes of Biomedical Sciences and Key Laboratory of Medical Molecular Virology Institute of Medical Microbiology Shanghai Medical College Fudan University China 2 Drug Discovery and Design Center State Key Laboratory of Drug Research Shanghai Institute of Materia Medica Chinese Academy of Sciences China 3 Shanghai Institute of Organic Chemistry Chinese Academy of Sciences China 4 State Key Laboratory of Molecular Biology and Research Center for Structural Biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences China Keywords crystal structure shikimate dehydrogenase shikimate pathway site-directed mutagenesis Staphylococcus epidermidis Correspondence D. Qu Institutes of Biomedical Sciences and Key Laboratory of Medical Molecular Virology Institute of Medical Microbiology Shanghai Medical College Fudan University Shanghai 200032 China Fax 86 21 54237603 Tel 86 21 54237524 E-mail dqu@ X. Shen Drug Discovery and Design Center State Key Laboratory of Drug Research Shanghai Institute of Materia Medica Chinese Academy of Sciences Shanghai 201203 China Fax Tel 86 21 50806918 E-mail xshen@ These authors contributed equally to this work Database Coordinate and structure factor files for SeSDH and SeSDH in complex with shikimate have been deposited in the Protein Data Bank under the accession numbers 3DON and 3DOO Received 28 August 2008 revised 9 November 2008 accepted 12 December 2008 doi Shikimate dehydrogenase SDH catalyzes the NADPH-dependent reduction of 3-dehydroshikimate to shikimate in the shikimate pathway. In this study we determined the kinetic .

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