TAILIEUCHUNG - Báo cáo khao học: "Survival after outplanting of the ectomycorrhizal fungus Laccaria bicolor S238N inoculated on Douglas fir (Pseudotsuga menziesii (Mirb.) Franco) cuttings"

Tuyển tập các báo cáo nghiên cứu về lâm nghiệp được đăng trên tạp chí lâm nghiệp quốc tế đề tài: Survival after outplanting of the ectomycorrhizal fungus Laccaria bicolor S238N inoculated on Douglas fir (Pseudotsuga menziesii (Mirb.) Franco) cuttings. | Ann. For. Sci. 59 2002 81-92 INRA EDP Sciences 2002 DOI forest 2001007 81 Original article Survival after outplanting of the ectomycorrhizal fungus Laccaria bicolor S238N inoculated on Douglas fir Pseudotsuga menziesii Mirb. Franco cuttings Céline Di Battistaa Daniel Bouchardb Francis Martinb Benoit Generec Jean-Michel Amiraultd and Franẹois Le Taconb aUnité Agronomie et Environnement UC 864 INRA ENSAIA 2 avenue de la Foret de Haye BP 172 54505 Vandreuvre-les-Nancy Cedex France bUnité Mixte de Recherches Interactions Arbres-Microorganismes INRA Centre de Nancy 54280 Champenoux France c Direction Départementale de l Agriculture et de la Foret Cité administrative 2 rue Saint-Sever 76032 Rouen France d CEMAGREF - Domaine des Barres 45290 Nogent-sur-Vernisson France Received 6 March 2001 accepted 14 September 2001 Abstract - Selected strains of ectomycorrhizal fungi can be inoculated in forest nurseries to improve survival and growth of seedlings or cuttings after field transplantation. The survival of the American strain Laccaria bicolor S238N on Douglas fir cuttings was evaluated in nursery and field conditions three years after outplanting using morphological and PCR RFLP of nuclear rDNA spacers. The comparison of the mycorrhizal status of Douglas fir cuttings at the end of the nursery phase and two years after outplanting shown several behaviours among the ectomycorrhizal fungi naturally occurring in the nursery or artificially introduced. The naturally occurring Rhizopogon type disappeared after outplanting while the inoculated strain Laccaria bicolor S238N and an unknown type 1 2 ITS ribotype survived and competed with the naturally occurring fungi of the outplanting site. Only one indigenous type 1 3 ITS ribotype seemed occurring in the outplanting site where Cenococcum geophilum was almost completely absent. Douglas fir mycorrhizas nursery transplantation Résumé - Survie après transplantation de la souche ectomycorhizienne Laccaria bicolor S238N .

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