TAILIEUCHUNG - Báo cáo khoa học: "Origin identification of maritime pine stands in France using chloroplast simple-sequence repeats"

Tuyển tập các báo cáo nghiên cứu về lâm nghiệp được đăng trên tạp chí lâm nghiệp quốc tế đề tài: Origin identification of maritime pine stands in France using chloroplast simple-sequence repeats. | Ann. For. Sci. 59 2002 53-62 INRA EDP Sciences 2002 DOI forest 2001005 53 Original article Origin identification of maritime pine stands in France using chloroplast simple-sequence repeats Maria Margarida Ribeiro 1 Grégoire Sophie Gerberb Giovanni Guiseppe Vendraminc Maria Anzideic Stéphane Decroocqb Anne Marpeaud Stéphanie Marietteb and Christophe Plomionb Department of Forest Genetics and Plant Physiology SLU 901 83 Umeả Sweden bINRA Équipe de Génétique et Amelioration des Arbres Forestiers BP 45 33610 Cestas France cIstituto Miglioramento Genetico Piante Forestali CNR Via Atto Vannucci 13 50134 Firenze Italy dLaboratoire de Chimie des Substances Végétales Institut du Pin Université de Bordeaux 1 351 cours de la libération 33405 Talence Cedex France Received 20 October 2000 accepted 28 February 2001 Abstract - Maritime pine seed-lots from north-western Iberian regions Portugal and Galicia were introduced in the 1950s to the southwest of France Aquitaine region and the stands they formed suffered considerable frost damage. In the mid 1980s a biochemical test was developed to test the putative origin of adult stands in Aquitaine before seeds could be distributed for commercial purposes in France. In this paper we describe a new test employing chloroplast simple-sequence repeats cpSSRs to facilitate identification of stand origin based on randomisation tests. The origin of five stands of unknown origin was determined with both the cpSSR and biochemical terpene profile analysis tests. The results from the two tests were concordant but the DNA-based test gave faster and more accurate results. Use of this test should help when determining the origin of maritime pine stands in the Aquitaine region of France. cpSSR microsatellites terpene origin identification Pinus pinaster Résumé - Identification de l origine géographique des peuplements de pin maritime en France à l aide de microsatellites chloro-plastiques. Des lots de graines du nord ouest de la

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