TAILIEUCHUNG - Báo cáo khoa học: Llama single-domain antibodies directed against nonconventional epitopes of tumor-associated carcinoembryonic antigen absent from nonspecific cross-reacting antigen

Single-domain antibodies (sdAbs), which occur naturally in camelids, are endowed with many characteristics that make them attractive candidates as building blocks to create new antibody-related therapeutic molecules. In this study, we isolated from an immunized llama several high-affinity sdAbs directed against human carcinoembryonic antigen (CEA), a heavily glycosylated tumor-associated molecule expressed in a variety of cancers. | ỊFEBS Journal Llama single-domain antibodies directed against nonconventional epitopes of tumor-associated carcinoembryonic antigen absent from nonspecific cross-reacting antigen í íhĩclaĩno Rohar1 2 Pa rii 1 f hamoc1 2 l Icahalla Toi I Inn2 3 Ạ nnól io Pnrn 11 Inn 1 2 Paĩca I Ạ le hn I ilzr2 4 5 kJ I 11 siaine Dclldl IdU K VI lai I ICO I loaucllc I euioi I - I I lelle VUII IIIIUI I rdloa I - l oliuu IM Francoise Roquet2 6 Martine Pugniere2 6 Jean-Luc Teillaud2 7 8 9 Anne Gruaz-Guyon2 4 5 Andre Pelegrin2 3 and Daniel Baty1 2 f 1 CNRS Laboratoire d Ingenierie des Systemes Macromoleculaires Marseille France 2 CNRS Groupement de Recherche Immunociblage des Tumeurs Marseille France 3 INSERM Centre de Recherche en cancerologie de Montpellier Universite Montpellier France 4 INSERM Centre de Recherche Biomedicale Bichat-Beaujon Paris France 5 Universite Denis Diderot-Paris 7 France 6 CNRS Universite Montpellier 1 France 7 INSERM Centre de Recherche des Cordeliers Paris France 8 Universite Pierre et Marie Curie - Paris 6 France 9 University Paris Descartes France Keywords carcinoembryonic antigen CEACAM5 nonspecific cross-reacting antigen phage display single domain antibodies Correspondence D. Baty INSERM U624 Stress Cellulaire Marseille France Fax 33 4 91 82 60 83 Tel 33 4 91 82 88 33 E-mail Present addresses UMR6204 CNRS Universite de Nantes France ỊINSERM U624 Stress Cellulaire Marseille France Database The nucleotide sequences in this study have been submitted to the GenBank database under the accession numbers ABS29543 C3 ABS29544 C17 ABS29545 C25 ABS29546 C43 and ABS29547 C44 Received 18 December 2008 revised 20 April2009 accepted 15 May 2009 doi Single-domain antibodies sdAbs which occur naturally in camelids are endowed with many characteristics that make them attractive candidates as building blocks to create new antibody-related therapeutic molecules. In this study we isolated from an immunized llama several

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