TAILIEUCHUNG - Báo cáo khoa học: Oocyte membrane localization of vitellogenin receptor coincides with queen flying age, and receptor silencing by RNAi disrupts egg formation in fire ant virgin queens

Oocyte membrane localization of vitellogenin receptor coincides with queen flying age, and receptor silencing by RNAi disrupts egg formation in fire ant virgin queens Hsiao-Ling Lu, S. B. Vinson and Patricia V. Pietrantonio Department of Entomology, Texas A&M University, College Station, TX, USA Keywords insect ovary; insect reproduction; oocyte development; RNA interference; social insects Correspondence P. V. Pietrantonio, Department of Entomology, Texas A&M University, College Station, TX 77843-2475, USA Fax: +1 979 845 6305 Tel: +1 979 845 9728 E-mail: p-pietrantonio@ Website: faculty/ (Received 13 March 2009, revised 27 March 2009, accepted 30 March 2009) doi: In ant species in which mating flights. | Oocyte membrane localization of vitellogenin receptor coincides with queen flying age and receptor silencing by RNAi disrupts egg formation in fire ant virgin queens Hsiao-Ling Lu S. B. Vinson and Patricia V. Pietrantonio Department of Entomology Texas A M University College Station TX USA Keywords insect ovary insect reproduction oocyte development RNA interference social insects Correspondence P. V. Pietrantonio Department of Entomology Texas A M University College Station tX 77843-2475 USA Fax 1 979 845 6305 Tel 1 979 845 9728 E-mail p-pietrantonio@ Website http people faculty Received 13 March 2009 revised 27 March 2009 accepted 30 March 2009 doi In ant species in which mating flights are a strategic life-history trait for dispersal and reproduction maturation of virgin queens occurs. However the specific molecular mechanisms that mark this transition and the effectors that control premating ovarian growth are unknown. The vitellogenin receptor VgR is responsible for vitellogenin uptake during egg formation in insects. In the red imported fire ant Solenopsis invicta Buren Hymenop-tera Formicidae virgin queens have more abundant VgR transcripts than newly mated queens but limited egg formation. To elucidate whether the transition to egg production involved changes in VgR expression we investigated both virgin and mated queens. In both queens western blot analysis showed an ovary-specific VgR band 202 kDa and immunofluorescence analysis of ovaries detected differential VgR localization in early- and latestage oocytes. However the VgR signal was much lower in virgin queens ready to fly than in mated queens 8 h post mating flight. In virgin queens the receptor signal was first observed at the oocyte membrane beginning at day 12 post emergence coinciding with the 2 weeks of maturation required before a mating flight. Thus the membrane localization of VgR appears to be a potential marker for

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