TAILIEUCHUNG - Báo cáo khoa học: Activator-binding domains of the SWI ⁄ SNF chromatin remodeling complex characterized in vitro are required for its recruitment to promoters in vivo

Interaction between acidic activation domains and the activator-binding domains of Swi1 and Snf5 of the yeast SWI⁄SNF chromatin remodeling complex has previously been characterized in vitro. Although deletion of both activator-binding domains leads to phenotypes that differ from the wild-type, their relative importance for SWI⁄SNF recruitment to target genes has not been investigated. | ỊFEBS Journal Activator-binding domains of the SWI SNF chromatin remodeling complex characterized in vitro are required for its recruitment to promoters in vivo Monica E. Ferreira1 2 Philippe Prochasson3 Kurt D. Berndt1 2 Jerry L. Workman3 and Anthony P. H. Wright1 2 1 Schoolof Life Sciences Sodertorns Hogskola Huddinge Sweden 2 Department of Biosciences and MedicalNutrition Karolinska Institutet Huddinge Sweden 3 Stowers Institute for MedicalResearch Kansas City MO USA Keywords chromatin remodeling coactivator recruitment SWI SNF complex transcriptionalactivation yeast Correspondence A. P. H. Wright Schoolof Life Sciences Sodertorns Hogskola S-141 89 Huddinge Sweden Fax 46 8 6084510 Tel 46 8 6084708 E-mail Present address Department of Pathology and Laboratory Medicine University of Kansas Medical Center KS USA Received 15 December 2008 revised 20 February 2009 accepted 23 February 2009 doi Interaction between acidic activation domains and the activator-binding domains of Swil and Snf5 of the yeast SWI SNF chromatin remodeling complex has previously been characterized in vitro. Although deletion of both activator-binding domains leads to phenotypes that differ from the wild-type their relative importance for SWI SNF recruitment to target genes has not been investigated. In the present study we used chromatin immunoprecipitation assays to investigate the individual and collective importance of the activator-binding domains for SWI SNF recruitment to genes within the GAL regulon in vivo. We also investigated the consequences of defective SWI SNF recruitment for target gene activation. We demonstrate that deletion of both activator-binding domains essentially abolishes galactose-induced SWI SNF recruitment and causes a reduction in transcriptional activation similar in magnitude to that associated with a complete loss of SWI SNF activity. The activator-binding domains in Swi1 and Snf5 make approximately equal .

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