TAILIEUCHUNG - Báo cáo sinh học: "The recombinant adeno-associated virus vector (rAAV2)-mediated apolipoprotein B mRNA-specific hammerhead ribozyme: a self-complementary AAV2 vector improves the gene expression"

Tuyển tập các báo cáo nghiên cứu về sinh học được đăng trên tạp chí sinh học Journal of Biology đề tài: The recombinant adeno-associated virus vector (rAAV2)-mediated apolipoprotein B mRNA-specific hammerhead ribozyme: a self-complementary AAV2 vector improves the gene expression | Genetic Vaccines and Therapy BioMed Central Research Open Access The recombinant adeno-associated virus vector rAAV2 -mediated apolipoprotein B mRNA-specific hammerhead ribozyme a self-complementary AAV2 vector improves the gene expression Shumei Zhong1 Shihua Sun1 and Ba-Bie Teng 1 2 Address 1Research Center for Human Genetics Institute of Molecular Medicine The University of Texas Health Science Center at Houston Houston TX 77030 and 2University of Texas Graduate School of Biomedical Sciences at Houston Houston TX 77030 Email Shumei Zhong - Shihua Sun - shihuas@ Ba-Bie Teng - Corresponding author Published II June 2004 Received 01 April 2004 Accepted 11 June 2004 Genetic vaccines and Therapy 2004 2 5 This article is available from http content 2 1 5 2004 Zhong et al licensee BioMed Central Ltd. This is an Open Access article verbatim copying and redistribution of this article are permitted in all media for any purpose provided this notice is preserved along with the article s original URL. Abstract Background In humans overproduction of apolipoprotein B apoB is positively associated with premature coronary artery diseases. To reduce the levels of apoB mRNA we have designed an apoB mRNA-specific hammerhead ribozyme targeted at nucleotide sequences GUA6679 RB15 mediated by adenovirus which efficiently cleaves and decreases apoB mRNA by 80 in mouse liver and attenuates the hyperlipidemic condition. In the current study we used an adeno-associated virus vector serotype 2 AAV2 and a self-complementary AAV2 vector scAAV2 to demonstrate the effect of long-term tissue-specific gene expression of RB15 on the regulation apoB mRNA in vivo. Methods We constructed a hammerhead ribozyme RB15 driven by a liver-specific transthyretin TTR promoter using an AAV2 vector rAAV2-TTR-RB15 . HepG2 cells and hyperlipidemic mice deficient in both the low density lipoprotein receptor and the apoB .

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