TAILIEUCHUNG - Báo cáo sinh học: "Localization of leucocyte interferon gene in the q2.5 region of pig chromosomel by in situ hybridization"

Tuyển tập các báo cáo nghiên cứu về sinh học được đăng trên tạp chí sinh học Journal of Biology đề tài: Localization of leucocyte interferon gene in the region of pig chromosomel by in situ hybridization | Genet. Sei. Evol. 1989 21 249-252 Elsevier INRA 249 Short communication Localization of leucocyte interferon gene in the region of pig chromosomel by in situ hybridization M. Yerle and J. Gellin Institut National de la Recherche Agronomique centre de recherches de Toulouse labora-toire de génétique cellulaire BP27 31326 Castanet-Tolosan Cedex France received 11 April 1989 accepted 5 May 1989 Summary - Using in situ hybridization and the random primer method to label the probe we reduced the region of localization of leukocyte interferon gene on pig chromosome 1 from to . pig - leukocyte interferon - in situ hybridization Resume Localisation precise par hybridation in situ d un gene interferon a sur le chromosome 1 du pore. L utilisation en hybridation in situ de la sonde correspondant au gene de I interferon a marquee par le systeme d amorces oligonuẹléotidiqụes a permis de réduire la zone de localisation de ce gene sur le chromosome 1 du pore de la region - à la region . pore - interferon a - hybridation in situ INTRODUCTION In 1986 we mapped the leukocyte interferon gene on pig chromosome 1 by in situ hybridization Yerle et al. 1986 . The labeled region - appeared to be large considering that the probe used contained only one alpha interferon gene. We thought that the probe could hybridize with the different alpha interferon genes that were supposed to present 80-95 homologies in nucleotide sequence as in man and mouse. Nevertheless after changing the labeling system of the probe we increased the precision of the localization as we reduced it to one band . MATERIALS AND METHODS The metaphase spreads were obtained from peripheral blood lymphocyte cultures established from normal male pigs. The metaphases were G banded GTG banding technique before hybridization and the best ones were photographed. The probe was a recombinant plasmid pUC8 containing a fragment of 2 700 bp of genomic pig DNA including an entire alpha .

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