TAILIEUCHUNG - Báo cáo sinh học: "Immediate transfection of patient-derived leukemia: a novel source for generating cell-based vaccines"

Tuyển tập các báo cáo nghiên cứu về sinh học được đăng trên tạp chí sinh học Journal of Biology đề tài: Immediate transfection of patient-derived leukemia: a novel source for generating cell-based vaccines | Genetic Vaccines and Therapy BioMed Central Research Open Access Immediate transfection of patient-derived leukemia a novel source for generating cell-based vaccines Jill A Gershan Bryon D Johnson James Weber Dennis W Schauer Natalia Natalia Stephanie Behnke Karen Burns Kelly W Maloney Anne B Warwick and Rimas J Orentas Address Department of Pediatrics Medical College of Wisconsin and the Children s Research Institute Children s Hospital of Wisconsin 8701 Watertown Plank Rd. Milwaukee WI 53226 USA Email Jill A Gershan - jgershan@ Bryon D Johnson - bjohnson@ James Weber - jweber@ Dennis W Schauer - dschauer@ Natalia Natalia - nnatalia@ Stephanie Behnke - sbehnke@ Karen Burns - kburns@ Kelly W Maloney - kmaloney@ Anne B Warwick - awarwick@ Rimas J Orentas - rorentas@ Corresponding author Published 21 June 2005 Received 26 March 2005 Genetic Vaccines and Therapy 2005 3 4 doi 186 1479-0556-3-4 Accepted 21 June 2005 This article is available from http content 3 1 4 2005 Gershan et al licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License http licenses by which permits unrestricted use distribution and reproduction in any medium provided the original work is properly cited. Abstract Background The production of cell-based cancer vaccines by gene vectors encoding proteins that stimulate the immune system has advanced rapidly in model systems. We sought to develop non-viral transfection methods that could transform patient tumor cells into cancer vaccines paving the way for rapid production of autologous cell-based vaccines. Methods As the extended culture and expansion of most patient tumor cells is not possible we sought to first evaluate a new technology that combines electroporation and chemical transfection in order to determine if plasmid-based

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