TAILIEUCHUNG - Báo cáo sinh học: "A functional genomic analysis of cell morphology using RNA interference"

Tuyển tập các báo cáo nghiên cứu về sinh học được đăng trên tạp chí sinh học Journal of Biology đề tài: A functional genomic analysis of cell morphology using RNA interference. | J. Biol. Journal of Biology BioMed Central Research article Open Access A functional genomic analysis of cell morphology using RNA interference AA Kiger B Baum S Jones MR Jones A Coulson C EcheverrH and N Perrimon Addresses Department of Genetics Harvard Medical School Howard Hughes Medical Institute Boston MA 02115 USA. Genome Sciences Centre British Columbia Cancer Research Centre Vancouver V5Z 4E6 Canada. MRC Laboratory of Molecular Biology Cambridge CB2 2QH UK. Cenix BioScience GmbH D-01307 Dresden Germany. Current address Ludwig Institute for Cancer Research University College London W1W 7BS UK. Correspondence Norbert Perrimon. E-mail perrimon@ Received 17 April 2003 Revised 17 July 2003 Accepted 12 August 2003 Published 1 October 2003 Journal of Biology 2003 2 27 The electronic version of this article is the complete one and can be found online at http content 2 4 27 2003 Kiger et al. licensee BioMed Central Ltd. This is an Open Access article verbatim copying and redistribution of this article are permitted in all media for any purpose provided this notice is preserved along with the article s original URL. Abstract Background The diversity of metazoan cell shapes is influenced by the dynamic cytoskeletal network. With the advent of RNA-interference RNAi technology it is now possible to screen systematically for genes controlling specific cell-biological processes including those required to generate distinct morphologies. Results We adapted existing RNAi technology in Drosophila cell culture for use in high-throughput screens to enable a comprehensive genetic dissection of cell morphogenesis. To identify genes responsible for the characteristic shape of two morphologically distinct cell lines we performed RNAi screens in each line with a set of double-stranded RNAs dsRNAs targeting 994 predicted cell shape regulators. Using automated fluorescence microscopy to visualize actin filaments microtubules and DNA we detected .

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