TAILIEUCHUNG - Báo cáo khoa học: Transcript profiling during the early development of the maize brace root via Solexa sequencing

Currently, the molecular regulation mechanisms involved in the early development of maize brace root are poorly known. To gain insight into the transcriptome dynamics that are associated with its development, genome-wide gene expression profiling was conducted by Solexa sequencing (Illumina Inc., San Diego, CA, USA). | IFEBS Journal Transcript profiling during the early development of the maize brace root via Solexa sequencing Yan-Jie Li Ya-Ru Fu Jin-Guang Huang Chang-Ai Wu and Cheng-Chao Zheng State Key Laboratory of Crop Biology College of Life Sciences Shandong AgriculturalUniversity China Keywords brace root differential expression maize Solexa sequencing tag Correspondence . Zheng or Wu College of Life Sciences Shandong AgriculturalUniversity Taian Shandong 271018 China Fax 86 538 8226399 Tel 86 538 8242894 86 538 8246678 E-mail cczheng@ cawu@ These authors contributed equally to this work Received 16 September 2010 revised 19 October 2010 accepted 26 October 2010 doi Currently the molecular regulation mechanisms involved in the early development of maize brace root are poorly known. To gain insight into the transcriptome dynamics that are associated with its development genome-wide gene expression profiling was conducted by Solexa sequencing Illumina Inc. San Diego CA USA . More than five million tags were generated from the stem node tissues without and with just-emerged brace roots including 149 524 and 178 131 clean tags in the two libraries respectively. Of these 82 864 and 91 678 tags were matched to the reference genes. The most differentially expressed tags with a log2 ratio 2 or -2 P were analyzed further representing 143 up-regulated and 152 down-regulated genes except for unknown transcripts which were classified into 11 functional categories. The most enriched categories were those of metabolism signal transduction and cellular transport. Many genes or biological pathways were found to be commonly shared between brace root and lateral or adventitious root development such as genes participating in cell wall degradation and synthesis auxin transport and signaling ethylene signaling etc. Next the expression patterns of 20 genes were assessed by quantitative real-time PCR and the results

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