TAILIEUCHUNG - Báo cáo khoa học: Identification of a putative triacylglycerol lipase from papaya latex by functional proteomics

Latex from Caricaceae has been known since 1925 to contain strong lipase activity. However, attempts to purify and identify the enzyme were not suc-cessful, mainly because of the lack of solubility of the enzyme. Here, we describe the characterization of lipase activity of the latex ofVasconcel-lea heilbornii and the identification of a putative homologous lipase from Carica papaya. | IFEBS Journal Identification of a putative triacylglycerol lipase from papaya latex by functional proteomics R. Dhouib1 J. Laroche-Traineau2 R. Shaha1 D. Lapaillerie3 E. Solier3 J. Ruales4 M. Pina5 P. Villeneuve5 F. Carriere1 M. Bonneu3 and V. Arondel1 2 1 CNRS Aix-Marseille Université Enzymologie Interfaciale et Physiologie de la Lipolyse France 2 Universite de Bordeaux CNRS UMR 5200 Laboratoire de Biogenese Membranaire France 3 Universite de Bordeaux Centre de Genomique Fonctionnelle France 4 Departamento de Ciencia de Alimentos y Biotechnologia Escuela Politecnica Nacional Quito Ecuador 5 UMR IATE Laboratoire de Lipotechnie CIRAD Montpellier Cedex 5 France Keywords Carica papaya latex lipase phospholipase A2 Vasconcellea heilbornii Correspondence V. Arondel Universite de Bordeaux CNRS UMR 5200 Laboratoire de Biogenese Membranaire 146 Rue Leo Saignat 33076 Bordeaux Cedex France Fax 33 556 518 361 Tel. 33 557 574 508 E-mail . Received 15 June 2010 revised 28 September 2010 accepted 25 October 2010 doi Latex from Caricaceae has been known since 1925 to contain strong lipase activity. However attempts to purify and identify the enzyme were not successful mainly because of the lack of solubility of the enzyme. Here we describe the characterization of lipase activity of the latex of Vasconcel-lea heilbornii and the identification of a putative homologous lipase from Carica papaya. Triacylglycerol lipase activity was enriched 74-fold from crude latex of Vasconcellea heilbornii to a specific activity SA of 57 imol-min_1-mg_1 on long-chain triacylglycerol olive oil . The extract was also active on trioctanoin SA 655 imol-min_1-mg_1 tributyrin SA 1107 imol-min_1-mg_1 and phosphatidylcholine SA 923 imol-min_1-mg_1 . The optimum pH ranged from to . The protein content of the insoluble fraction of latex was analyzed by electrophoresis followed by mass spectrometry and 28 different proteins were .

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