TAILIEUCHUNG - Báo cáo sinh học: " Cloning and expression profiling of the VLDLR gene associated with egg performance in duck (Anas platyrhynchos)"

Tuyển tập các báo cáo nghiên cứu về sinh học được đăng trên tạp chí sinh học quốc tế đề tài: Cloning and expression profiling of the VLDLR gene associated with egg performance in duck (Anas platyrhynchos) | Wang et al. Genetics Selection Evolution 2011 43 29 http content 43 1 29 Ge n et i cs Selection Evolution RESEARCH Open Access Cloning and expression profiling of the VLDLR gene associated with egg performance in duck Anas platyrhynchos Cui Wang Shi-jun Li Wen-hua Yu Qing-wu Xin Chuang Li Yan-ping Feng Xiu-li Peng and Yan-zhang Gong Abstract Background The very low density lipoprotein receptor gene VLDLR a member of the low density lipoprotein receptor LDLR gene family plays a crucial role in the synthesis of yolk protein precursors in oviparous species. Differential splicing of this gene has been reported in human rabbit and rat. In chicken studies showed that the VLDLR protein on the oocyte surface mediates the uptake of yolk protein precursors into growing oocytes. However information on the VLDLR gene in duck is still scarce. Methods Full-length duck VLDLR cDNA was obtained by comparative cloning and rapid amplification of cDNA ends RACE . Tissue expression patterns were analysed by semi-quantitative reverse-transcription polymerase chain reaction RT-PCR . Association between the different genotypes and egg performance traits was investigated with the general linear model GLM procedure of the SAS software package. Results In duck two VLDLR transcripts were identified one transcript variant-a containing an O-linked sugar domain and the other variant-b not containing this sugar domain. These transcripts share 70 to 90 identity with their counterparts in other species. A phylogenetic tree based on amino acid sequences showed that duck VLDLR proteins were closely related with those of chicken and zebra finch. The two duck VLDLR transcripts are differentially expressed . VLDLR-a is mainly expressed in muscle tissue and VLDLR-b in reproductive organs. We have localized the duck VLDLR gene on chromosome Z. An association analysis using two completely linked SNP sites T C at position 2025 bp of the ORF and G A in intron 13 and records from two .

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