TAILIEUCHUNG - Báo cáo sinh học: "ChickRH6: a chicken whole-genome radiation hybrid panel"

Tuyển tập các báo cáo nghiên cứu về sinh học được đăng trên tạp chí sinh học thế giới đề tài: ChickRH6: a chicken whole-genome radiation hybrid panel | 521 Genet Sei. Evol. 34 2002 521-533 INRA EDP Sciences 2002 DOI gse 2002021 Original article ChickRH6 a chicken whole-genome radiation hybrid panel Mireille Morisson Alexandre Lemière Sarah Bosc Maxime Galan Florence Plisson-Petit Philippe Pinton Chantal Delcros Katia Fève Frederique Pitel Valerie Fillon Martine Yerle Alain Vignal Laboratoire de génétique cellulaire Institut national de la recherche agronomique 31326 Castanet-Tolosan France Received 4 February 2002 accepted 13 May 2002 Abstract - Asa first step towards the development of radiation hybrid maps we have produced a radiation hybrid panel in the chicken by fusing female embryonic diploid fibroblasts irradiated at 6 000 rads with HPRT-deficient hamster Wg3hCl2 cells. Due to the low retention frequency of the chicken fragments a high number of clones was produced from which the best ones were selected. Thus 452 fusion clones were tested for retention frequencies with a panel of 46 markers. Based on these results 103 clones with a mean marker retention of were selected for large scale culture to produce DNA in sufficient quantities for the genotyping of numerous markers. Retention frequency was tested again with the same 46 markers and the 90 best clones with a final mean retention frequency of were selected for the final panel. This panel will be a valuable resource for fine mapping of markers and genes in the chicken and will also help in building BAC contigs. chicken radiation hybrid mapping 1. introduction The interest in studying the chicken genome has greatly increased in the past years driving international efforts towards detailed physical and genetic maps for a review see 29 . One of the reasons for this interest is the importance of this species in agriculture. The various current efforts aiming at mapping QTLs quantitative trait loci involved in production traits and the resistance to pathogens will beneht greatly from detailed knowledge of the genome. Other reasons include the

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