TAILIEUCHUNG - Báo cáo khoa học: Saccharomyces cerevisiae Cox18 complements the essential Sec-independent function of Escherichia coli YidC

Members of the YidC⁄Oxa1⁄Alb3 protein family function in the biogenesis of membrane proteins in bacteria, mitochondria and chloroplasts. InEsc-herichia coli, YidC plays a key role in the integration and assembly of many inner membrane proteins. | ễFEBS Journal Saccharomyces cerevisiae Cox18 complements the essential Sec-independent function of Escherichia coli YidC Edwin van Bloois1 Gregory Koningstein1 Heike Bauerschmitt2 3 Johannes M. Herrmann2 and Joen Luirink1 1 Department of Molecular Microbiology Institute of Molecular CellBiology Vrije Universiteit Amsterdam the Netherlands 2 Zellbiologie Technische Universitat Kaiserslautern Germany 3 Institut fur Physiologische Chemie Universitat Munchen Germany Keywords Cox18 Oxa1 SecYEG signal recognition particle YidC Correspondence J. Luirink Department of Molecular Microbiology Institute of Molecular Cell Biology Vrije Universiteit De Boelelaan 1085 1081 HV Amsterdam the Netherlands Fax 31-20-5987155 Tel 31-20-5987175 E-mail Received 27 July 2007 revised 31 August 2007 accepted 4 September 2007 Members of the YidC Oxa1 Alb3 protein family function in the biogenesis of membrane proteins in bacteria mitochondria and chloroplasts. In Escherichia coli YidC plays a key role in the integration and assembly of many inner membrane proteins. Interestingly YidC functions both in concert with the Sec-translocon and as a separate insertase independent of the translocon. Mitochondria of higher eukaryotes contain two distant homologues of YidC Oxa1 and Cox18 Oxa2. Oxa1 is required for the insertion of membrane proteins into the mitochondrial inner membrane. Cox18 Oxa2 plays a poorly defined role in the biogenesis of the cytochrome c oxidase complex. Employing a genetic complementation approach by expressing the conserved region of yeast Cox18 in E. coli we show here that Cox18 is able to complement the essential Sec-independent function of YidC. This identifies Cox18 as a bona fide member of the YidC Oxa1 Alb3 family. doi Conserved mechanisms are used for the targeting and assembly of membrane proteins in bacteria mitochondria and chloroplasts. This is illustrated by the conserved YidC Oxa1 Alb3 protein family that .

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