TAILIEUCHUNG - Báo cáo khoa học: Optical-fiber bundles

Optical-fiber bundles have been employed as a versatile substrate for the fabrication of high-density microwell arrays. In this minireview, we discuss the application of optical-fiber-bundle arrays for a variety of biological problems. | MINIREVIEW Optical-fiber bundles Hans H. Gorris Timothy M. Blicharz and David R. Walt Department of Chemistry Tufts University Medford MA USA Keywords array artificial olfaction bead cell DNA lab-on chip optical-fiber bundle single molecule Correspondence D. Walt Department of Chemistry Tufts University 62 Talbot Avenue Medford MA 02155 USA Fax 1 617 627 3443 Tel 1 617 627 3470 E-mail Received 9 July 2007 accepted 29 August 2007 Optical-fiber bundles have been employed as a versatile substrate for the fabrication of high-density microwell arrays. In this minireview we discuss the application of optical-fiber-bundle arrays for a variety of biological problems. For genomics studies and microbial pathogen detection individual beads have been functionalized with DNA probes and then loaded into the microwells. In addition beads differentially responsive to vapors have been employed in an artificial olfaction system. Microwell arrays have also been loaded with living cells to monitor their individual response to biologically active compounds over long periods. Finally the microwells have been sealed to enclose single enzyme molecules that can be used to measure individual molecule catalytic activity. doi Introduction The focus of biological research has shifted considerably during the last few years. Traditionally biomolecules such as DNA RNA peptides proteins lipids or carbohydrates have been identified and characterized one by one by isolating and purifying the molecule of interest. Although this procedure remains important it is not practical for gaining a comprehensive view of the myriad processes in living systems. In the post-genomic area it has become apparent that the linear DNA sequence of a genome holds only a small fraction of the information required for understanding whole organisms. The need to take a broader picture represents the motivation for systems biology 1 . The need to make many measurements .

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