TAILIEUCHUNG - Báo cáo khoa học: Induction of translationally controlled tumor protein (TCTP) by transcriptional and post-transcriptional mechanisms

Expression of the humanTPT1gene coding for translationally controlled tumor protein (TCTP) was investigated in Calu-6 and Cos-7 cells under the influence of 4b-phorbol 12-myristate 13-acetate (PMA), forskolin, dioxin and the heavy metals copper, nickel and cobalt. | ễFEBS Journal Induction of translationally controlled tumor protein TCTP by transcriptional and post-transcriptional mechanisms Irina Schmidt Michael Fahling Benno Nafz Angela Skalweit and Bernd-Joachim Thiele Charites Universitatsmedizin Berlin Institut fur Vegetative Physiologie Germany Keywords gene expression post-transcriptional control TPT1 gene transcription translationally controlled tumor protein TCTP Correspondence . Thiele Charites Universitatsmedizin Berlin Institut fur Vegetative Physiologie Tucholskystr. 2 10117 Berlin Germany Fax 49 30 450 528972 Tel 49 30 450 528184 E-mail Received 14 June 2007 revised 23 August 2007 accepted 28 August 2007 doi Expression of the human TPT1 gene coding for translationally controlled tumor protein TCTP was investigated in Calu-6 and Cos-7 cells under the influence of 4p-phorbol 12-myristate 13-acetate PMA forskolin dioxin and the heavy metals copper nickel and cobalt. Transcriptional and post-transcriptional aspects of the mechanism were analyzed by TCTP mRNA protein quantification luciferase reporter gene assays depending on TPT1 promoter sequences or TCTP mRNA 5 3 -UTRs and investigation of the interaction of RNA-binding proteins with UTRs by UV-cross-linking. PMA forskolin dioxin cobalt and nickel induced TCTP expression in 24 h in both cell lines about at the mRNA level and at the protein level. The highest induction rate at the mRNA level and at the protein level was observed with copper. TPT1 promoter assays showed transcriptional activation by PMA forskolin and dioxin and a increase by copper whereas cobalt and nickel had no effect. Deletion analysis revealed that copper-dependent transcriptional control was transmitted by a metal-responsive element residing in the TPT1 promoter. Post-transcriptional activation of TCTP expression was associated with the action of dioxin .

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