TAILIEUCHUNG - Báo cáo y học: "The role of F1 ATP synthase beta subunit in WSSV infection in the shrimp, Litopenaeus vannamei"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: The role of F1 ATP synthase beta subunit in WSSV infection in the shrimp, Litopenaeus vannamei | Liang et al. Virology Journal 2010 7 144 http content 7 1 144 VIROLOGY JOURNAL RESEARCH Open Access The role of F1 ATP synthase beta subunit in WSSV infection in the shrimp Litopenaeus vannamei Yan Liang Jun-Jun Cheng Bing Yang Jie Huang Abstract Background Knowledge of the virus-host cell interaction could inform us of the molecular pathways exploited by the virus. Studies on viral attachment proteins VAPs and candidate receptor proteins involved in WSSV infection allow a better understanding of how these proteins interact in the viral life cycle. In this study our aim was to find some host cellular membrane proteins that could bind with white spot syndrome virus WSSV . Results Two proteins were evident by using a virus overlay protein binding assay VOPBA with WSSV. A protein with molecular weight 53 kDa named BP53 was analyzed in this study which was homologous with the F1-ATP synthase beta subunit by mass spectrometry analysis. Rapid amplification of cDNA ends RACE PCR was performed to identify the full-length cDNA of the bp53 gene. The resulting full-length gene consisted of 1836 bp encoding 525 amino acids with a calculated molecular mass of kDa. The deduced amino acid sequence contained three conserved domains of the F1-ATP synthase beta subunit. BP53 was therefore designated the F1-ATP synthase beta subunit of L. vannamei. The binding of WSSV to BP53 were also confirmed by competitive ELISA binding assay and co-immunoprecipitation on magnetic beads. To investigate the function of BP53 in WSSV infection it was mixed with WSSV before the mixture was injected intramuscularly into shrimp. The resulting mortality curves showed that recombinant r BP53 could attenuate WSSV infection. Conclusions The results revealed that BP53 is involved in WSSV infection. Here is the first time showed the role of shrimp F1-ATP synthase beta subunit in WSSV infection. Background White Spot Syndrome Virus WSSV is a species in the newly described genus .

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