TAILIEUCHUNG - Báo cáo y học: " Serodiagnosis of sheeppox and goatpox using an indirect ELISA based on synthetic peptide targeting for the major antigen P32"

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Serodiagnosis of sheeppox and goatpox using an indirect ELISA based on synthetic peptide targeting for the major antigen P32 | Tian et al. Virology Journal 2010 7 245 http content 7 1 245 VIROLOGY JOURNAL RESEARCH Open Access Serodiagnosis of sheeppox and goatpox using an indirect ELISA based on synthetic peptide targeting for the major antigen P32 Hong Tian Yan Chen Jinyan Wu Youjun Shang Xiangtao Liu Abstract Background Sheeppoxvirus SPPV goatpoxvirus GTPV and lumpy skin disease virus LSDV of cattle belong to the Capripoxvirus genus of the Poxviridae family and can cause significant economic losses in countries where they are endemic. Despite the considerable threat that these viruses pose to livestock production and global trade in sheep goats cattle and their products convenient and effective serodiagnostic tools are not readily available. Toward this goal two synthetic peptides corresponding to the major antigen P32 were synthesized. These synthetic peptides were then used as antigen to develop an ELISA method to detect anti-SPPV and GTPV antibodies. Results The results indicated that the optimal concentration of coated recombinant antigen was pg per well for a serum dilution of 1 10. The ELISA performed favorably when sera from sheep immunized experimentally were tested. Conclusion This assay offers the prospect of synthetic peptide as antigens for indirect ELISA to detect SPPV and GTPV antibody in sheep and goat sera. Background Goat pox GP and sheep pox SP are malignant diseases of small ruminants causing heavy economic loss in the endemic countries. The diseases are endemic in India Bangladesh throughout the near and middle east northern and central Africa 1 2 . The causative agents sheep pox and goat pox viruses belong to the genus Capripoxvirus in the family Poxviridae 3 4 . Although experienced veterinarians readily diagnose these diseases in their acute forms low virulence strains and other exanthemas in sheep . orf or scabby mouth Parapoxviridae can present problems for differential diagnosis. Laboratory confirmation has been reliant upon classical .

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