TAILIEUCHUNG - Isolation of lactic acid bacteria from traditional moroccan products and evaluation of their antifungal activity on growth and ochratoxin a production by Aspergillus carbonarius and A. niger

These results revealed strain-dependent antifungal activity. Our study indicates that the use of these lactic acid bacteria can be applied as a biocontrol of fungal growth and mycotoxins production. | ISOLATION OF LACTIC ACID BACTERIA FROM TRADITIONAL MOROCCAN PRODUCTS AND EVALUATION OF THEIR ANTIFUNGAL ACTIVITY ON GROWTH AND OCHRATOXIN A PRODUCTION BY ASPERGILLUS CARBONARIUS AND A. NIGER Yousra El HAMMOUDI 1 2 Adil LAAZIZ1 Amina BOUSETA1 and Rajae BELKHOU2 Address es Yousra EL HAMMOUDI 1 Laboratory of Biotechnology Environment Agri-food and Health Sidi Mohamed Ben Abdallah University Faculty of Science Dhar Mahraz . 1796 Atlas Fez Morocco. 2 Laboratory of Bio-industry and Agro-food Technology University Sidi Mohamed Ben Abdallah Higher School of Technology Fez Route d Imouzzer 2427 - Fez Morocco. Corresponding author https ARTICLE INFO ABSTRACT Received 25. 8. 2020 Different agricultural products including grapes are susceptible to contamination and damage by several microorganisms one being Revised 21. 1. 2021 fungi and their mycotoxins such as ochratoxin A. These alterations are a source of food waste and lead to significant economic losses. Accepted 25. 2. 2021 Thus new alternatives have been sought in parallel with existing methods. In this work after isolation identification by 16S rDNA Published 1. 8. 2021 sequencing and differentiation by Pulsed-Field Gel Electrophoresis PFGE of four lactic acid bacteria from different traditional Moroccan food products antifungal activity was studied against Aspergillus carbonarius and A. niger isolated from Moroccan grapes. Their effect on ochratoxin A production was also assessed. A strain of Lactoccocus lactis ssp. lactis F and three strains of Leuconostoc Regular article mesenteroides ssp. mesenteroides I C and D selected from 16 isolated strains were tested. Their antifungal activities against Aspergillus spp. were quite significant with growth inhibition rates ranging from to . Likewise ochratoxin A production by Aspergillus strains was significantly reduced. Indeed in the presence of the Lactoccocus lactis ssp. lactis F strain this .

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