TAILIEUCHUNG - Optimization, purification and characterization of phytase from isolated probiotic Pediococcus acidilactici BNS5B

Microbial phytases are of immense importance due to their application in food/feed industry by enhancing the availability of essential minerals such as phosphorous, iron, calcium etc. required for normal human physiology and also have commercial and environment significance. Therefore, in present study an attempt was made to enhance the production of phytase from isolated probiotic Pediococcus acidilactici BNS5B by employing both one variable at a time approach and statistically based design of experiments such as Plackett-Burman and Response Surface Methodology. Interestingly, phytase production was enhanced 94 fold at an optimised condition of galactose, yeast extract, beef extract and ammonium sulphate. Further, the phytase enzyme was purified and had apparent molecular weight of 43 KDa, pH optima of with pH stability in the range of , temperature optima of 40°C and retaining an activity of 76% at a temperature range of 20-80°C and followed normal Michael-Menten curve with the kinetic parameter Km and Vmax of and respectively. | Optimization, purification and characterization of phytase from isolated probiotic Pediococcus acidilactici BNS5B

• Hoá học
• Plackett Burman design
• Response surface methodology
• Ionexchange chromatography
• Pediococcus acidilactici BNS5B
• Purification and characterization
• Journal of Chemistry
• Partial purification
• Biochemical characterization
• Lipolytic enzymes
• Enzyme purification
• Production of an antibacterial compound
• Purification and characterization of an antibacterial compound
• Streptomyces griseus TBG19NRA1
• Antimicrobial activities
• Streptomyces griseus
• Vietnam Journal of Science and Technology
• The fungal strain FBV40
• Purification and characterization of laccase involved
• The white rot fungus isolated
• Bavi forest of Vietnam
• Thioredoxin reductase
• Characterization purification
• Rainbow trout
• Metal ions
• Achatina achatina
• Sodium carboxymethyl cellulose
• Gel filtration
• Dinitrosalicyclic acid
• Labeo rohita
• Acidic proteases
• Alkaline proteases
• Enzyme purification fold
• Enzyme kinetics
• BMC Biotechnology
• Recombinant human tyrosyl tRNA synthetase
• Expression of rhTyrRS
• Protein purification
• Biological activity assay
• Protein expression and purification
• Pichia pastoris
• Autoimmune diseases
• Biochemical properties
• Carica papaya
• Pectin lyase
• Papaya pericarp
• Characterization of two pectinases
• Extracellular glutaminase free l asparaginase
• Trichoderma viride F2
• Mitigating acrylamide formation
• Starchy fried food
• Aureobasidium pullulans
• Intracellular fructosyltransferase
• Original activity
• Enzyme preparations
• Positional specificity
• Papaver somniferum
• Biologically active
• Pseudomonas fluorescens
• Extracellular lipase
• P nitrophenyl esters
• Bacterial enzymes
• báo cáo khoa học
• báo cáo y học
• công trình nghiên cứu về y học
• tài liệu về y học
• cách trình bày báo cáo
• tài liệu báo cáo nghiên cứu khoa học
• kiến thức bệnh thú y
• báo cáo bệnh thú y
• các nghiên về bệnh thú ý
• báo cáo hóa học
• công trình nghiên cứu về hóa học
• tài liệu về hóa học
• Autothermal Thermophilic
• Aerobic Digestion
• Agro Industrial Wastes
• olid State
• Fermentation Processes
• Pozzolanic Materials
• Antifungal protein
• Serratia marcescens DT3
• Serine 3 hydroxylase
• Remarkable yield loss
• Fusarium oxysporum
• Pichia pastoris X 33
• Recombinant protein
• Recombinant human paraoxonase
• Saccharomyces cerevisiae alpha factor signal sequence
• High density lipoprotein
• Anoxybacillus kestanbolensis AC26Sari
• Isopropyl β D thiogalactopyranoside
• Inducible T7 promoter
• Thermophilic ribulokinase
• D ribulose activity
• Soluble expression
• Human tissue plasminogen activator
• Treat thrombosis
• Bacillus vallismortis
• 16S rDNA
• Solvent tolerant
• Heavy metal