TAILIEUCHUNG - Condition optimization for phosphate solubilization by Kosakonia cowanii MK834804, an endophytic bacterium isolated from Aegle marmelos

In the present study endophytic bacteria were isolated from Aegle marmelos and were screened for phosphate solubilizing activity. The isolate AP01 showed the ability to solubilize inorganic tricalcium phosphate and thus was characterized. Phenotypic, biochemical and genotypic analysis revealed, the isolate to be Kosakonia cowanii, a gram –ve proteobacterium of family enterobacteriaceae with accession no. MK834804, of the three media viz. Pikovskaya’s agar (PKVA), National Botanical Research Institute’s phosphate growth medium (NBRIP) and NBRIP-BPB (Bromo Phenol Blue) agar media tested for Phosphate solubilizing efficiency, the isolate showed high Phosphate solubilizing Index (PSI ) in PKVA medium. Whereas, under submerged fermentation the isolate showed highest phosphate solubilization in NBRIP medium () on 4 th day with maximum drop down of medium pH (). Optimum solubilization of phosphorus was observed at temperature 37ºC (), pH 8 ( µg/ml) with % ammonium sulphate () and 2% Lactose () on 4th day of incubation period as optimal nitrogen and carbon sources respectively. Release of various organic acids . oxalic acid, malic acid, tartaric acid and gluconic acid into the medium was characterized by High Performance Liquid Chromatography (HPLC), during phosphate solubilization by the isolate. | Condition optimization for phosphate solubilization by Kosakonia cowanii MK834804, an endophytic bacterium isolated from Aegle marmelos

• Sinh học
• Phosphate solubilization
• Aegle marmelos
• Kosakonia cowanii
• Tricalcium phosphate
• Organic acid
• In vitro phosphate solubilization
• Sugarcane (Saccharum officinarum)
• Rhizosphere bacteria
• Suitable conditions were
• Aluminium phosphate
• Ferric phosphate
• Days after inoculation
• Organic acids
• Phosphate solubilization Rhizobacteria
• Osmotolerant bacteria
• Osmotic stress
• Organic acid production profile
• Tricalcium phosphate solubilizatio
• Phosphorus fetilizers
• Rock phosphorus
• Spent wash
• Low grade udaipur rajasthan rock phosphate
• Spent wash from Faizabad
• Phytohormones production
• Phosphate solubilization capacities
• Fluorescent Pseudomonas sp
• Apple rhizosphere
• Indigenous fluorescent Pseudomonas sp
• Enterobacter cancerogenus
• Potato plant
• Phosphate solubilizing bacteria
• Rhizosphere of potato plant
• Zea mays L
• Vegetative growth parameters
• Cultivable microbial diversity
• Characterization of phosphate solubilizers
• Rock phosphate
• Total sugar and total starch
• Wheat in an Inceptisol
• Levels of phosphorus
• Rock phosphate on protein
• Indole 3 acetic acid
• Pot trials
• Plant growth–promoting rhizobacteria
• Pikovsakaya’s medium
• Paddy rhizosphere soil
• Isolation and screening
• Solubilization efficiency
• Phosphorus solubilization
• Isolation screening
• Phosphate solubilizing fungi
• Soybean Rhizosphere
• Produced cellulase enzyme
• P solubilization
• Pikovskaya’s agar
• Selection of phosphate solubilizing fungi
• Maize rhizosphere
• Lactic acid bacteria
• Microbial consortia
• Healthy spinach (Spinacia oleracea)
• Lactic acid
• Enterobacter hormachei
• NBRIP broth
• Phosphate solubilisation
• Carbon source
• Nitrogen source
• Phosphate solubalizing microorganisms
• Plant growth promoters
• Mechanism of P solubilization
• Growing demand of food
• Aluminum phosphate
• Cluster bean
• Biofertilizer and sustainable agriculture
• Rhizobial strains isolated
• Cluster bean plant native
• Antimicrobial activity
• Plant growth promoting bacteria
• Teucrium polium L
• Endophyte bacteria
• Fruit yield
• Lysinibacillus sp isolate 1TH16a
• IAA production
• Endophytic bacteria
• Nitrogenase enzyme
• Azospirillium sp
• Plant growth promotion
• Wheat genotypes
• Growth of chickpea
• Chickpea (Pisum sativum L )
• Plant under saline condition
• Saline condition
• Macrophomina phaseolina
• Biocontrol potential
• Plant growth promoting effect
• Agricultural science
• The roots of potato plant
• Acromobacter sp
• Arachis hypogea
• Plant growth promoting rhizobacteria
• PVK broth
• Physico chemical analysis