TAILIEUCHUNG - Morphological development and ultrastructural changes during somatic embryogenesis of popular banana cultivars

Somatic embryogenesis based plant regeneration is a pre-requisite for genetic transformation of banana. Mass multiplication of banana through somatic embryogenesis is also viewed as an economically viable alternative to commonly used shoot tip based method. Banana somatic embryogenesis response was studied in three popular banana cultivars, Grand Naine, Rasthali and Hill banana „Virupakshi‟. Immature male flower bud explant was used for embryogenic callus induction. Different morphological types of calli were produced during callus induction that includes both embryogenic and nonembryogenic calli. White translucent pro-embryogenic calli were found to be suitable for establishment of embryogenic cell suspension (ECS). Regeneration potential of ECS was assessed and the development of different stages of embryo viz., globular, scutellum, and coleoptillar stages was observed under scanning electron microscope to understand their ultra-structure. The differences in the duration of developmental stages and regeneration potential could be attributed to the genomic differences of cultivars involved. | Morphological development and ultrastructural changes during somatic embryogenesis of popular banana cultivars

• Sinh học
• Somatic embryogenesis
• Embryogenic calli
• Non embryogenic calli
• Cell suspension cultures
• Somatic embryo
• Scanning electron microscope
• Grape hyacinths
• Muscari armeniacum
• Ornamental plants
• BMC Genomics
• Somatic tissue
• In vitro
• Growth regulators
• Phylogenetic analysis
• Arabidopsis thaliana proteins
• Digitalis lamarckii Ivan
• Dwarf foxglove
• Cotyledonary leaf segments
• Callus formation
• Shoot organogenesis
• Journal of Biotechnology
• Primary and secondary somatic embryogenesis
• Jatropha curcas L
• Leaf transverse thin cell layers
• Secondary embryos considerably
• Sub cultured onto B5
• Gene expression
• Leaf explants
• Generate embryogenic cells
• Dwarfing cherry rootstock
• Tylophora indica
• Leaf callus
• Murashige and Skoog
• Merr
• via leaf explants
• BMC Plant Biology
• Theobroma cacao
• Cell reprogramming
• Plant propagation
• Transient gene expression
• Zygotic embryogenesis
• Hevea brasiliensis
• RNA seq
• Hormone signal
• Transcription factor
• Histone modification
• Vegetative propagation
• Myrtaceae family
• Widely planted hardwood
• Differentially expressed genes
• B3 superfamily
• Callus initiation
• Expression profile
• Dimocarpus longan
• Illumina HiSeq
• Auxin and cytokinin
• Molecular marker gene
• Early somatic embryogenesis
• Illumina HiSeq sequencing
• Long non coding RNAs
• Microspore embryogenesis
• Anther culture
• DNA methylation
• 5 methyl deoxy cytidine
• Walnut Juglans regia L
• Immature cotyledon
• Apomictic seed
• Walnut genotype
• Chestnut Castanea sativa Mill
• Castanea sativa Mill
• Driver and Kuniyuki walnut
• Embryogenic cotyledon
• Zygotic embryo
• Grape rootstock
• Vitis vinifera L
• Regenerated somatic plantlets
• Plant tissue culture
• Wheat genetic
• Plant regeneration
• Established in soil
• Translucent structures
• Embryogenic structures
• 2 4 D and kinetin
• Initiation of somatic embryogenesis
• Silver nanoparticles enhanced efficiency
• Explant surface disinfection
• Embryogenesis in begonia tuberous via thin cell layer culture
• Micropropagation of B tuberous
• Eliminating infectious microorganisms
• Simultaneous shoot
• Fast regeneration
• Root induction
• Sugarcane Saccharum officinarum L
• Red clover
• Transmission electron microscope
• Kinetin trials
• Aseptic seedlings
• Plant growth regulators
• Embryogenic potential
• Pseudotsuga menziesii
• Cotyledonary somatic embryos
• Tissue culture
• Leaf as explants
• Overcome conventional limitations