TAILIEUCHUNG - Indirect somatic embryogenesis and shoot organogenesis from cotyledonary leaf segments of Digitalis lamarckii Ivan., an endemic medicinal species
This study describes, for the first time, an in vitro protocol for the development of somatic embryos and shoots from a callus derived from cotyledonary leaf segments excised from 3-week-old in vitro-germinated seedlings of Digitalis lamarckii Ivan. (dwarf foxglove), an endemic medicinal species of Turkey. | S. K. VERMA, B. B. YÜCESAN, S. GÜREL, E. GÜREL Turk J Biol 35 (2011) 743-750 © TÜBİTAK doi: Indirect somatic embryogenesis and shoot organogenesis from cotyledonary leaf segments of Digitalis lamarckii Ivan., an endemic medicinal species Sandeep Kumar VERMA1, Bahtiyar Buhara YÜCESAN1, Songül GÜREL2, Ekrem GÜREL1 1 Department of Biology, Abant İzzet Baysal University, 14280 Bolu - TURKEY 2 Department of Plant Breeding, Sugar Institute, 06790 Etimesgut, Ankara - TURKEY Received: Abstract: This study describes, for the first time, an in vitro protocol for the development of somatic embryos and shoots from a callus derived from cotyledonary leaf segments excised from 3-week-old in vitro-germinated seedlings of Digitalis lamarckii Ivan. (dwarf foxglove), an endemic medicinal species of Turkey. The embryogenic callus was induced on Murashige and Skoog (MS) medium containing μM α-naphthalene acetic acid (NAA) and μM 6-benzylaminopurine (BAP). Somatic embryos developed readily when the embryogenic callus was transferred to MS medium containing BAP ( or μM) alone or BAP combined with NAA (, , or μM). The most effective hormonal combination for somatic embryogenesis was μM NAA and μM BAP, which produced a mean of embryos per cotyledonary leaf explant. An organogenic callus was induced on MS medium containing μM NAA and μM BAP. Shoot development was observed when the organogenic callus was transferred to MS medium containing different concentrations of BAP alone (, , or μM) or BAP combined with NAA ( or μM). The highest mean number of shoots ( shoots per explant) was obtained when the medium contained μM BAP and μM NAA. The regenerated shoots were readily rooted on MS medium containing , , or 5 μM indole-3-acetic acid (IAA) or indole-3-butyric acid (IBA). Rooted regenerants were then transferred to the pots, where they grew well and attained
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