TAILIEUCHUNG - Somatic embryogenesis and encapsulation of immature bulblets of an ornamental species, grape hyacinths (Muscari armeniacum Leichtlin ex Baker)
An efficient in vitro regeneration system was developed for the ornamental Muscari armeniacum on Linsmaier and Skoog basal medium (LS) supplemented with benzyladenine (BA) at varying concentrations (, , or mg/L) alone or in combination with mg/L α-naphthalene acetic acid (NAA). | Turkish Journal of Agriculture and Forestry Turk J Agric For (2014) 38: 716-722 © TÜBİTAK doi: Research Article Somatic embryogenesis and encapsulation of immature bulblets of an ornamental species, grape hyacinths (Muscari armeniacum Leichtlin ex Baker) 1, 1 2 2 Bahtiyar Buhara YÜCESAN *, Feride ÇİÇEK , Ekrem GÜREL Department of Seed Science and Technology, Faculty of Natural and Agricultural Sciences, Abant İzzet Baysal University, Bolu, Turkey 2 Department of Biology, Faculty of Science and Literature, Abant İzzet Baysal University, Bolu, Turkey Received: Accepted: Published Online: Printed: Abstract: An efficient in vitro regeneration system was developed for the ornamental Muscari armeniacum on Linsmaier and Skoog basal medium (LS) supplemented with benzyladenine (BA) at varying concentrations (, , or mg/L) alone or in combination with mg/L α-naphthalene acetic acid (NAA). The highest mean number of direct somatic embryo formations was observed on LS medium containing mg/L BA and mg/L NAA, with a mean of somatic embryos per explant after 10 weeks of culture. Green nodular calli induced on LS medium containing mg/L BA alone or in combination with mg/L NAA were transferred to LS medium supplemented with or without mg/L gibberellic acid (GA3) for 8 weeks, producing immature bulblets. Immature bulblets produced in vitro were either embedded in a sodium alginate matrix for the encapsulation process or were transferred directly to LS medium supplemented with or without GA3 at or mg/L for growth and development for 6 weeks. Encapsulated bulblets were then stored at 4 °C in darkness for 10 weeks and almost all encapsulated bulblets retained their viability and resumed their growth under nonaxenic greenhouse conditions. Key words: Encapsulation, Muscari armeniacum, ornamental plants, somatic .
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