TAILIEUCHUNG - Purification and bioactivity of fucoidan from Kappaphycus alvarezii Alga

Kappaphycus alvarezii is a type of seaweed containing numerous carbohydrates with biological activities, especially fucoidans. In terms of chemical aspect, fucoidansare fucose polysaccharides (FCSPs) with diverse biological activities including antioxidant, anticoagulant, anti-inflammatory, antiviral, antitumor. Fucoidan is found not only in brown algae, but also in K. alvarezii alga from the central coast of Viet Nam. In this study, fucoidan from K. alvarezii alga (collected at Dam Mon area, Khanh Hoa province) was purified, its structure was characterized, and its biological activities were investigated. The recovery yield of two-stage precipitation by 99 % ethanol was %. The protocol of fucoidan purification by ion-exchange chromatography was applied with tris-HCl buffer, elution solvent was NaCl 1M; The yield was %. The structure of fucoidan from K. alvarezii from the second fraction was determined by 1H-NMR spectrum with the specific characteristic properties of fucoidan. The antioxidant activity of the purified fucoidan via IC50 was ± μg/mL (DPPH assay), and ± μg/mL (ABTS essay). The purified fucoidan exhibited better ability in cases of anticoagulant, antibacterial activities than that of the crude extract. The anti-coagulation time of the purified fucoidan was minutes. The minimum inhibitory concentrations (MICs) of the antibacterial activity were 400 μg/mL (Bacillus cereus, Escherichia coli), 500 μg/mL (Aspergillus flavus), and 600 μg/mL (Aspergillus niger). | Purification and bioactivity of fucoidan from Kappaphycus alvarezii Alga

• Hoá học
• Ion exchange chromatography
• Purification and bioactivity
• Fucoidan from Kappaphycus alvarezii Alga
• Kappaphycus alvarezii Alga
• Biological activities were investigated
• Chromatography A
• Ionexchange chromatography
• On line pH meter
• Monoclonal antibody
• Charge variants
• Plackett Burman design
• Response surface methodology
• Pediococcus acidilactici BNS5B
• Purification and characterization