TAILIEUCHUNG - Initial characterisation of exosomes released by umbilical cord-derived mesenchymal stem cells and mature dendritic cells, under ‘Good Manufacturing Practice’ conditions

Exosomes represent an important mode of intercellular communication and play key roles in many physiological and pathological processes. Exosomes have hitherto exhibited their capacity to modulate biological activities through their carrying of functional molecules such as proteins, lipids, and genetic materials. In the current study, we investigated exosomes released by mature dendritic cells (mDCs) and umbilical cord-derived mesenchymal stem cells (UCMSCs) under Good Manufacturing Practice (GMP) conditions. Ultracentrifugation was used to isolate and purify the exosomes. Additionally, a transmission electron microscope (TEM) and immunoblotting were used to characterise exosomal morphology and markers. The preliminary results showed that both mDCs and UCMSCs secreted exosomes into GMP culture media. Exosomes exhibited a cup-shaped morphology and showed positive for CD63. Additionally, no difference was observed between mDC-derived exosomes and UCMSC-derived exosomes regarding marker expression or morphology. This data indicates the potential for further development of GMP exosomes for clinical application. | Initial characterisation of exosomes released by umbilical cord-derived mesenchymal stem cells and mature dendritic cells, under ‘Good Manufacturing Practice’ conditions

• Sinh học
• Dendritic cells
• Mesenchymal stem cells
• Exosomes released
• Umbilical cord derived mesenchymal
• Mature dendritic cells
• trình bày báo cáo
• tài liệu báo cáo khoa học
• báo cáo y học
• kiến thức y học
• nghiên cứu y học
• Domestic animal species
• Skin of domestic animals
• Functional significance
• Dendritic cells in the skin
• Regulation of Apoptosis by IL 10
• Apoptosis by IL 10
• The association with Stat 1
• Signaling molecule in dendritic cells
• The priming role of dendritic cells
• The cancer cytotoxic effects of cytokine induced killer cells
• The priming role
• Granulocyte macrophage colony stimulating factor
• CIK cells interacted
• Dendritic cell
• Fas ligand
• Vitamin E
• Dendritic cells through PI3K signaling
• Fasl induced apoptotic death
• BMC Cancer
• Laryngeal cancer
• CD1a positive dendritic cells
• Cell cycle
• Cytokine induced killer cells
• Hepatocellular carcinoma
• Caspase 3
• Liver cancer stem cells
• C FLIP
• Vitamin C
• Mouse dendritic cells
• Journal of Biology
• Helicobacter felis
• Regulatory B cells
• Anti inflammatory cytokines
• Helicobacter pylori (H pylori)
• báo cáo khoa học
• công trình nghiên cứu về y học
• tài liệu về y học
• cách trình bày báo cáo
• Migration capability
• Sca1+ mesenchymal stromal cells
• Microenvironment regulates
• Tumor infiltrating dendritic cell
• Immune tolerance
• Mixed lymphocyte reaction
• Tumor microenvironment
• Clinical research
• Cytokine Induced Killer cell
• Advanced renal cancer
• BMC Genomics
• Infectious bronchitis virus
• Avian infectious bronchitis virus
• Neutralising viruses
• Plasmacytoid dendritic cells
• The murine discs large homolog 2
• Cell membranes
• Signaling enzymes
• Genome Biology
• Histone modifications
• Transcription factors
• Time series
• BMC Genetics
• Gene expression analysis
• Next generation sequencing
• báo cáo hóa học
• công trình nghiên cứu về hóa học
• tài liệu về hóa học
• Dendritic cell vaccine
• Hepatitis B virus
• Autoimmune diseases
• Quantitative RT PCR analysis
• Humoral immune response
• CD40 signalling
• Preclinical cancer models
• Therapeutic combination
• Modulated electro hyperthermia
• Hyperthermia field
• Cytotoxic T cell
• Single cell RNA seq
• Single cell genomics
• Elite controller
• Differential expression
• Schistosoma mansoni antigens
• Experimental schistosomiasis
• SWAP antigens
• Vaccination in mice experimentally infected