TAILIEUCHUNG - Regulation of Apoptosis by IL-10 and the association with Stat-1 signaling molecule in dendritic cells

IL-10 is an anti-inflammatory cytokine, participating in induction of immune tolerance and cell apoptotic death. Dendritic cells (DCs) is the most professional antigen-presenting cells among innate immune cells to exert generation and maintenance of immunological memory mediated through activation of T and B lymphocytes. The STAT signalling pathway plays a regulatory role of maturation and differentiation of immune cells. In this study, DCs were treated with inflammatory cytokines including TNF- , INF , IL-2 and IL-10 and subsequently examined the phosphorylation of STAT-1 and STAT-3, TNF-α concetration in cell suspension and the proportion of Annexin V+ and caspase 3+ cells. Methods used for this investigation include western blotting, flow cytometry and ELISA. DCs were derived from mouse bone marrow cells and cultured with GM-CSF for 8 days. As a result, IL-10, but not other cytokines enhanced the number of Annexin V+ cells and caspase 3 activity in DCs. More importantly, IL-10 also increased the phosphorylation of STAT-1 as well as the release of TNF-α into cell suspension. In conclusion, activation of STAT-1 might relate to the cell apoptotic death and TNF-α sectetion in IL-10-treated DCs. | Regulation of Apoptosis by IL-10 and the association with Stat-1 signaling molecule in dendritic cells

• Sinh học
• Regulation of Apoptosis by IL 10
• Apoptosis by IL 10
• The association with Stat 1
• Signaling molecule in dendritic cells
• Dendritic cells
• Mesenchymal stem cells
• Exosomes released
• Umbilical cord derived mesenchymal
• Mature dendritic cells
• trình bày báo cáo
• tài liệu báo cáo khoa học
• báo cáo y học
• kiến thức y học
• nghiên cứu y học
• Domestic animal species
• Skin of domestic animals
• Functional significance
• Dendritic cells in the skin
• The priming role of dendritic cells
• The cancer cytotoxic effects of cytokine induced killer cells
• The priming role
• Granulocyte macrophage colony stimulating factor
• CIK cells interacted
• Dendritic cell
• Fas ligand
• Vitamin E
• Dendritic cells through PI3K signaling
• Fasl induced apoptotic death
• BMC Cancer
• Laryngeal cancer
• CD1a positive dendritic cells
• Cell cycle
• Cytokine induced killer cells
• Hepatocellular carcinoma
• Caspase 3
• Liver cancer stem cells
• C FLIP
• Vitamin C
• Mouse dendritic cells
• Journal of Biology
• Helicobacter felis
• Regulatory B cells
• Anti inflammatory cytokines
• Helicobacter pylori (H pylori)
• báo cáo khoa học
• công trình nghiên cứu về y học
• tài liệu về y học
• cách trình bày báo cáo
• Migration capability
• Sca1+ mesenchymal stromal cells
• Microenvironment regulates
• Tumor infiltrating dendritic cell
• Immune tolerance
• Mixed lymphocyte reaction
• Tumor microenvironment
• Clinical research
• Cytokine Induced Killer cell
• Advanced renal cancer
• BMC Genomics
• Infectious bronchitis virus
• Avian infectious bronchitis virus
• Neutralising viruses
• Plasmacytoid dendritic cells
• The murine discs large homolog 2
• Cell membranes
• Signaling enzymes
• Genome Biology
• Histone modifications
• Transcription factors
• Time series
• BMC Genetics
• Gene expression analysis
• Next generation sequencing
• báo cáo hóa học
• công trình nghiên cứu về hóa học
• tài liệu về hóa học
• Dendritic cell vaccine
• Hepatitis B virus
• Autoimmune diseases
• Quantitative RT PCR analysis
• Humoral immune response
• CD40 signalling
• Preclinical cancer models
• Therapeutic combination
• Modulated electro hyperthermia
• Hyperthermia field
• Cytotoxic T cell
• Single cell RNA seq
• Single cell genomics
• Elite controller
• Differential expression
• Schistosoma mansoni antigens
• Experimental schistosomiasis
• SWAP antigens
• Vaccination in mice experimentally infected