TAILIEUCHUNG - Chapter 4: Hybrid molecules

Concept of hybrid molecules: When double strand DNA is steamed to a temperature exceed the melting temperature (Tm), it will separate into 2 single strands DNA due to breaks of H bonds. If the reaction temperature is then decreased slowly plus other appropriate experimental conditions, these ssDNA will pair again. This phenomenon is called the hybridization of molecules. Characteristic of the hybrid molecules: Specificity, the re-pairing occurs only between two complementary sequences. | LAI PHAÂN TÖÛ (MOLECULAR HYBRIDIZATION) CHÖÔNG 4 Concept of hybrid molecules When double strand DNA is steamed to a temperature exceed the melting temperature (Tm), it will separate into 2 single strands DNA due to breaks of H bonds. If the reaction temperature is then decreased slowly plus other appropriate experimental conditions, these ssDNA will pair again. This phenomenon is called the hybridization of molecules. Characteristic of the hybrid molecules: Specificity, the re-pairing occurs only between two complementary sequences. These complementary sequences can be DNA or RNA, leading to the formations of DNA-DNA, RNA-RNA or hybrid DNA-RNA TYPES OF HYBRIDIZATION - Hybrid in liquid phase (Lai trong pha loûng) - Hybrid on solid phase (Lai treân pha raén) - in situ hybridization (Lai taïi choã) - Southern Blot - Northern Blot - Western Blot First described by E. M. Southern in 1975. Applications of Southern hybridization RFLP’s, VNTR’s (Variable Number Tandem Repeat) and DNA .

• Sinh học
• the hybrid molecules
• phenomenon is called
• types of hybridization
• single strands DNA
• the reaction temperature
• concept of hybrid molecules
• BMC Cancer
• Cancer inhibitors
• Classification of cancer inhibitors
• Non inhibitors
• Active substructure
• Active functional groups
• Potency score
• BMC Chemistry
• Benzimidazole pyrazole
• Anti ulcer
• Autodock vina
• Benzimidazole pyrazole hybrid molecules
• Elemental analysis
• Journal of Chemistry
• Triazolyl benzotriazole
• Antimicrobial and antioxidant activities
• Klebsiella pneumoniae strain
• Aspergillus niger strain
• Nitrogen containing heterocycle
• Gastrointestinal toxicity
• Cyclooxygenase 1 and 2
• Carrageenan induced paw edema
• Nonsteroidal antiinflammatory drugs
• Benzothiophene–rhodanine hybrids