TAILIEUCHUNG - Construction of a genetic linkage map of citrus with random amplified polymorphic DNA (RAPD) markers using a progeny population from a complex intergeneric cross

A genetic linkage map of the progeny population was constructed with these RAPD markers. The map contains 63 markers distributed into 9 linkage groups, which possibly correspond to the 9 haploid chromosomes of Citrus. The total maximum length of the linkage map generated in this study was cM, with an average map distance of cM between markers. | Turk J Bot 31 (2007) 79-86 © TÜB‹TAK Research Article Construction of a Genetic Linkage Map of Citrus with Random Amplified Polymorphic DNA (RAPD) Markers Using a Progeny Population from a Complex Intergeneric Cross 1, 2 Mehtap fiAH‹N ÇEV‹K *, Gloria A. MOORE 1 Department of Horticultural Sciences, Faculty of Agriculture, Süleyman Demirel University, 32260 Isparta - TURKEY 2 Department of Horticultural Sciences, Plant Molecular and Cellular Biology Program, University of Florida, Gainesville, FL, 32611, USA Received: Accepted: Abstract: This study included 30 plants that were randomly selected from a larger progeny population obtained from a complex intergeneric cross of {C. grandis (L.) Osb. x [C. paradisi Macf. x Poncirus trifoliata (L.) Raf.]} x {[(C. paradisi Macf. x P. trifoliata (L.) Raf.) x C. reticulata Blanco] x [(C. paradisi Macf. x Poncirus trifoliata (L.) Raf.) x C. sinensis (L.) Osb.]}. Genomic DNA was extracted from leaf samples of these plants and analysed for polymorphisms by polymerase chain reaction (PCR), using 10-mer random primers. A total of 111 random amplified polymorphic DNA (RAPD) markers were identified using 38 random primers. A genetic linkage map of the progeny population was constructed with these RAPD markers. The map contains 63 markers distributed into 9 linkage groups, which possibly correspond to the 9 haploid chromosomes of Citrus. The total maximum length of the linkage map generated in this study was cM, with an average map distance of cM between markers. Key Words: Citrus, DNA markers, RAPD markers, genetic linkage mapping Rastgele Ço¤alt›lm›fl DNA (RAPD) Markörleri ve Cinsler Aras› Karmafl›k Melez Populasyonu Kullan›larak Turunçgil Genetik Haritas›n›n Oluflturulmas› Özet: Cinsler aras› kamafl›k {C. grandis (L.) Osb. x [C. paradisi Macf. x Poncirus trifoliata (L.) Raf.]} x {[(C. paradisi Macf. x P. trifoliata (L.) Raf.) x C. reticulata Blanco] x [(C. paradisi Macf. x Poncirus trifoliata .

• Nông nghiệp
• DNA markers
• RAPD markers
• Genetic linkage mapping
• Poncirus trifoliata L
• C
• reticulata Blanco
• Triticum durum
• Simple sequence repeat DNA markers
• DNA fingerprinting
• DNA sequence flanking
• Eukaryotic genomes
• Labeo rohita
• River ganga
• Microsatellite markers
• Genetic diversity
• Microsatellite DNA markers
• Molecular markers
• Diverse molecular markers
• Enormous world
• Genome mapping
• DNA polymorphism
• DNA BIOLOGY
• DNA EXTRACTION
• COMMERCIAL KITS
• STUTTER PRODUCTS
• DNA ANALYSIS
• MICROBIAL FORENSICS
• Allium sativum L
• Genetic characterization
• Maintenance of redundant garlic
• Duplicated genotypes
• DNA based markers
• Barley and wheat
• Simple sequence repeat
• PCR based marker system
• DNA barcode Orchids
• Molecular identification orchidaceae
• Molecular phylogeny
• Genetic similarity coefficient
• 19 standard reference rice varieties
• SSR markers
• True hybrids
• Confirmation of hybridity
• DNA based markers is essential
• Essential in chickpea
• Confirmation of hybridity in chickpea
• Y chromosome analysis
• Y chromosomespecific microsatellite DNA markers
• Chloroform method
• Modern geographical locations
• Andhra Pradesh
• Telangana with SSR markers
• DNA Isolation
• RAPD primers
• Random amplified polymorphic DNA
• Heat tolerance chickpea
• Molecular characterization
• Cicer arietinum L
• Molecular characterisation of DNA
• DNA of brown plant hopper biotypes
• Brown plant hopper biotypes
• Brown plant hopper resistance
• Various rice genotypes at gangavathi
• DNA barcodes
• ITS rDNA gene
• Phylogenetic tree
• Truong Sa archipelago
• Durum wheat
• Aegilops sp
• Polymorphic AFLP markers
• Polymerase chain reaction
• Green gram genotypes
• Morphological markers
• Cross transferability
• Expressed sequence tags
• Touch down PCR
• Expressed microsatellite
• Short tandem repeats
• BMC Plant Biology
• Aegilops umbellulata
• Aegilops tauschii
• RNA sequencing
• Molecular diversity
• Soybean genotypes
• Physiological characteristics
• Turkish Journal of Agriculture and Forestry
• Tạp chí Nông Lâm nghiệp
• Bulk segregant analysis
• Marker assisted selection
• Sequence characterized amplified regions
• Sex discrimination
• Nile tilapia fish
• Oreochromis niloticus
• Genetical sex
• Identification of genetic polymorphism
• Molecular biology techniques
• Sequence characterised amplified regions
• Chickpea genotypes
• Cetyltrimethylammonium bromide
• Almond varieties
• Root knot nematode
• DNA marker
• Almond rootstocks
• F1 crossbreed almonds