TAILIEUCHUNG - Menstrual cycles, endogenous sex hormones, chromosome sensitivity, sister chromatid exchange, chromosome aberrations

Mutation breeding techniques in combination with tissue culture and molecular marker methods provide a powerful tool for improvement of vegetatively propagated plants. | Turkish Journal of Biology Turk J Biol (2013) 37: 716-725 © TÜBİTAK doi: Research Article In vitro mutagenesis of Etlingera elatior (Jack) and early detection of mutation using RAPD markers 1 1,2, 1 3 Muhamad Fahmi YUNUS , Maheran Abd AZIZ *, Mihdzar Abdul KADIR , Siti Khalijah DAUD , Azmi Abdul RASHID 1 Department of Agriculture Technology, Faculty of Agriculture, Universiti Putra Malaysia, Serdang, Selangor, Malaysia 2 Laboratory of Plantation Crops, Institute of Tropical Agriculture, Universiti Putra Malaysia, Serdang, Selangor, Malaysia 3 Department of Biology, Faculty of Science, Universiti Putra Malaysia, Serdang, Selangor, Malaysia Received: Accepted: Published Online: 1 Printed: Abstract: Mutation breeding techniques in combination with tissue culture and molecular marker methods provide a powerful tool for improvement of vegetatively propagated plants. The aim of this study was to develop a protocol for shoot regeneration and mutation induction of Etlingera elatior. The results of irradiation on in vitro buds of E. elatior showed LD50 to be 10 Gy, with the survival of explants being sharply reduced at this dosage. All 8 selected gamma irradiated regenerants were differentiated from the untreated control based on the banding patterns obtained using 9 primers, which generated 59 reproducible bands, whereby 35 () were found to be polymorphic. Jaccard’s coefficient of similarity values ranging from to were indicative of the level of genetic variation among the mutants studied. For comparison between the potential lines (PL) and the control, a maximum similarity value () was observed in PL1 mutant, while the minimum value () was observed in PL7. In summary, a combination of irradiation, regeneration, multiplication, and random amplification of polymorphic DNA (RAPD) analysis for early screening of mutants can speed up .

• Sinh học
• Etlingera elatior
• Genetic variation
• Potential line
• Random amplification of polymorphic DNA
• Vegetatively propagated plants