TAILIEUCHUNG - Salinity response of transgenic potato genotypes expressing the oxalate oxidase gene

In vitro and in vivo responses of transgenic and non-transgenic potato (Solanum tuberosum L.) genotypes to salinity were investigated. A gene responsible for the production of oxalate oxidase enzyme, whose expression has been associated with plant stress tolerance, was used. | Turk J Agric For 29 (2005) 187-195 © TÜB‹TAK Salinity Response of Transgenic Potato Genotypes Expressing the Oxalate Oxidase Gene Hakan TURHAN Field Crops Department, Agricultural Faculty, Çanakkale Onsekiz Mart University, 17020, Çanakkale - TURKEY Received: Abstract: In vitro and in vivo responses of transgenic and non-transgenic potato (Solanum tuberosum L.) genotypes to salinity were investigated. A gene responsible for the production of oxalate oxidase enzyme, whose expression has been associated with plant stress tolerance, was used. Transgenic potato plants expressing the oxalate oxidase enzyme were produced using Agrobacteriummediated transformation. The transgenic nature of the plants was verified by their antibiotic (kanamycin) resistance and then in vitro enzyme assays. Four independent transgenic genotypes obtained from 2 cultivars, Maris Bard and Desiree, were tested at 5 levels of NaCl (0%, , , and w/v) in vitro. The same genotypes were also tested at 3 levels of NaCl (0%, and w/v) under glasshouse conditions (in vivo). The results revealed that the growth of all genotypes was significantly inhibited by increasing salt treatments under both in vitro and in vivo conditions. Transgenic genotypes showed a relatively higher salinity tolerance than the non-transgenic genotypes in vitro. On the other hand, in the glasshouse, the results were less consistent but some transgenic genotypes showed superior tuber yield characteristics to the non-transgenics under salt stress. Key Words: Enzyme, Oxalate oxidase, salinity, Solanum tuberosum Oksalat Oksidaz Geni Tafl›yan Transgenik Patates Genotiplerinin Tuzlulu¤a Yan›tlar› Özet: Bu çal›flmada, transgenik ve transgenik olmayan patates (Solanum tuberosum L.) genotiplerinin in vitro ve in vivo koflullarda tuzlulu¤a karfl› yan›tlar› araflt›r›lm›flt›r. Bitkide sentezlenmesi stres ile ilgili olan oksalat oksidaz enzimi üretiminden sorumlu olan oksalat oksidaz geni .

• Nông nghiệp
• Potato Solanum tuberosum L
• genotypes
• Oxalate oxidase
• Solanum tuberosum
• Oxidase enzyme
• Plant stress tolerance
• Potato (Solanum tuberosum L )
• Development of specific micronutrient formulation
• Yield of potato
• Yield of potato (Solanum tuberosum L )
• Phyllosphere bacteria
• Exploring phyllosphere bacteria
• Growth promotion
• Recommended dose of fertilizers
• Mustard oil cake
• Kufri Ashoka
• Socio economic profile
• Potato crop
• Solanum tuberosum L
• Farm women in potato field
• Potato production point
• Organic mulching
• Inorganic mulches
• Yield characteristics of potato
• Effect of nitrogen growth
• Nitrogen growth
• Potato (Solanum tuberosum L
• Wheat and maize
• Foliar application
• Zinc and Manganese
• Growth parameters
• Yield and Potato
• Irrigation schedule
• Soil enzyme activities
• Amino acid biostimulants
• Potato (Solanum tuberosum L ) crop
• Activated charcoal
• In vitro
• Potato varieties
• Activated charcoal on in vitro growth
• In situ green manuring
• B C ratio
• Economics of potato
• Important inm practice
• Clonal progenies
• Alternaria blight
• Clonal progenies of potato
• Screening for alternaria blight
• Coloured potato genotypes
• Association among morphological characters
• Path analysis
• Morphological characters
• Coloured potato (Solanum tuberosum L )
• Genetic divergence
• Cluster analysis
• Genetic divergence analysis in potato
• Genetic divergence analysis
• Tissue culture
• Micro propagation
• Tissue culture of potato
• In vitro micro propagation
• Calcium metalosate
• Late blight
• Tuber number and yield
• Quality parameters of potato
• Organic manures
• Organic carbon
• Integrated nutrient management
• Growth and yield attributes of potato
• Character association
• Path coefficient studies
• Path coefficient studies for yield
• Components in potato
• Soil available nutrients
• Leaf tissue N
• P and K
• Yield attributes in potato
• Potato yield
• Drip irrigation and fertigation
• NPK concentration and uptake
• NPK concentration
• Potato (Solanum tuberosum L ) yield
• Anther culture
• Responding anthers
• Doubled haploids
• Green regenerant yield
• Gamma radiation
• In vitro mutagenesis
• Mutation breeding
• Salt stress
• Genetic variability
• Genetic advance
• Yield and it’s attributing traits
• Genetic variability studies for yield
• Sustainable soil health management
• Sustainable soil health management of potato
• Influenced by Bio fertilizers
• Bio fertilizers
• Effect of foliar application
• Foliar application of nutrients on growth
• Nutrients on growth
• Quality of potato (Solanum tuberosum L )
• Surface sterilization
• Nodal cuttings
• MS media
• Kufri Bahar