TAILIEUCHUNG - Evaluation of the genetic relationship between Fritillaria species from Turkey’s flora using fluorescent-based AFLP

In this study, the genetic relationships among 12 Fritillaria taxa collected from different regions of Turkey were investigated using the amplified fragment length polymorphism (AFLP) technique. | Turkish Journal of Biology Turk J Biol (2013) 37: 273-279 © TÜBİTAK doi: Research Article Evaluation of the genetic relationship between Fritillaria species from Turkey’s flora using fluorescent-based AFLP 1, 1 1, 2 3 Özge KARAKAŞ METİN *, Mine TÜRKTAŞ **, Meral ASLAY , Erdal KAYA TÜBİTAK Marmara Research Center, Genetic Engineering and Biotechnology Institute, Gebze, Kocaeli, Turkey 2 Erzincan Central Horticultural Research Station, Erzincan, Turkey 3 Atatürk Central Horticultural Research Institute, Yalova , Turkey Received: Accepted: Published Online: Printed: Abstract: In this study, the genetic relationships among 12 Fritillaria taxa collected from different regions of Turkey were investigated using the amplified fragment length polymorphism (AFLP) technique. Seven primer pair combinations were used for the analysis. The neighbor-joining and principal coordinate analysis methods were implemented to identify the taxonomy of the species. The analyses generated congruent results with each other and with the previous studies. The neighbor-joining analysis clustered the species into 3 groups, while the principal coordinates analysis did not support separation of the third group. The subgenera Fritillaria and Petilium diverged from each other with strong support in all analyses. The neighbor-joining tree further divided the members of subgenus Fritillaria into 2 subgroups. Statistical analysis of the data set using NTSYS showed a very good fit between the data matrix and the cophenetic matrix for the AFLP data with an r-value of . Generating a high polymorphic band profile, this analysis presented the feasibility of using AFLP for a genetic relationship analysis of Fritillaria. This primary report on AFLP analysis of Fritillaria species could facilitate achieving a deeper understanding of genetic relationships between the species. Key words: .

• Sinh học
• Amplified fragment length polymorphism
• Neighbor joining
• Genetic relationships
• Polymerase chain reaction
• Erzincan horticultural central research institute
• Cucumis sativus L
• Zucchini yellow mosaic virus
• Molecular markers
• Cucurbit crops including cucumber
• Sequence related amplified polymorphism
• Genetic relatedness
• Genotype specific bands
• Random priming
• Quantitative traits loci
• Sequence characterized amplified region
• trình bày báo cáo
• tài liệu báo cáo khoa học
• báo cáo bệnh thú y
• kiến thức bênh thú y
• nghiên cứu bệnh thú y
• báo cáo khoa học
• báo cáo y học
• công trình nghiên cứu về y học
• tài liệu về y học
• cách trình bày báo cáo
• Genetic resources
• Leaf antioxidant properties
• Genetic diversity
• Genetic structure
• Nuclear DNA content
• Lycopersicon esculentum Mill
• Droplet vitrification
• Genetic stability
• LCY B gene
• Plant Biology
• Genetic linkage
• Population structure
• MLM model
• Marker stability
• Turkish Journal of Agriculture and Forestry
• Tạp chí Nông Lâm nghiệp
• Bayesian cluster analysis
• Principal component analysis
• Croatian carob tree
• Trifolium L
• Genetic relationship
• Randomamplified polymorphic DNA
• Rhodiola rosea
• Simple sequence repeat
• sorghi
• Plant–microbe interaction
• CDNA AFLP
• Differential gene expression
• BMC Plant Biology
• QTL mapping
• Seed traits
• Seed size
• Seed coat color
• Single nucleotide polymorphism
• Blue honeysuckle
• Lonicera caerulea
• Asymmetry indexes
• Phenotype heterogeneity
• Restriction fragment length polymorphism
• Random amplified polymorphic DNA