TAILIEUCHUNG - Development of AFLP markers associated with zucchini yellow mosaic virus resistance in cucumber (Cucumis sativus L.)

Zucchini yellow mosaic virus (ZYMV) is one of the most important pathogens that cause significant yield losses in many cucurbit crops including cucumber (Cucumis sativus L). ZYMV resistance in cucumber is inherited by a single recessive gene. The purpose of this study was to identify molecular markers linked to the gene conferring ZYMV resistance in cucumber. | Turkish Journal of Botany Turk J Bot (2015) 39: 982-987 © TÜBİTAK doi: Research Article Development of AFLP markers associated with zucchini yellow mosaic virus resistance in cucumber (Cucumis sativus L.) 1,* 1 1 2 Hasan Özgür ŞIĞVA , Ahmet Fikret FIRAT , Gülden HAZARHUN , Ahmet İPEK 1 May-Agro Seed Corp., Bursa, Turkey 2 Department of Horticulture, Faculty of Agriculture, Uludağ University, Görükle Campus, Bursa, Turkey Received: Accepted/Published Online: Printed: Abstract: Zucchini yellow mosaic virus (ZYMV) is one of the most important pathogens that cause significant yield losses in many cucurbit crops including cucumber (Cucumis sativus L). ZYMV resistance in cucumber is inherited by a single recessive gene. The purpose of this study was to identify molecular markers linked to the gene conferring ZYMV resistance in cucumber. We developed a population of 188 F2 plants derived from inbred cucumber lines. Individual F2 plants were self-pollinated to generate F3 populations. Ten randomly selected plants from each F3 population were tested for ZYMV resistance. We used a bulk segregant analysis method to identify putative molecular markers linked to ZYMV resistance. Using bulked DNA samples with parental lines and F1, a total of 170 sequence-related amplified polymorphism (SRAP), 586 simple sequence repeat (SSR), and 308 amplified fragment length polymorphism (AFLP) primer combinations were screened. Neither polymorphic SRAP nor SSR markers were linked with ZYMV resistance. Among the 308 AFLP primer combinations tested, an AFLP marker in the E-ACA/MCA primer combination showed significant association among parental lines, F1, and resistant and susceptible plants. The combination of E-ACA/M-CA was achieved on parental lines, F1, and 188 F2 individuals for confirmation of the marker segregation on the F2 population. We found that the combination of E-ACA/M-CA was .

• Nông nghiệp
• Cucumis sativus L
• Zucchini yellow mosaic virus
• Molecular markers
• Cucurbit crops including cucumber
• Amplified fragment length polymorphism
• Sequence related amplified polymorphism
• Cucumber (Cucumis sativus L
• Genetic advance
• Genetic variability in cucumber
• Variance revealed significant
• Evaluation of cucumber
• Cucumber (Cucumis sativus L )
• Cucumber (Cucumis sativus L ) genotypes
• Hill zone of Karnataka
• Sirsi Local 2 exhibited superiority
• The amount of irrigation water for cucumber
• Cucumis sativus L grown on four types of substrate
• The amount of irrigated water
• The highest absolute yield
• Fungal pathogens associated
• Spoilage of cucumber
• Cucumber (Cucumis sativus L ) fruits
• Fungal isolates
• Agromorphological traits
• Cucumis sativus
• Pollen grains
• Palynological studies in Cucumber
• Gene effects
• Generation mean analysis
• Gene effects for fruit yield
• Calcium humate
• Boron humate
• Boron uptake of tomato
• Tomato (Lycopersicon esculentum L
• Relative water content
• Superoxide dismutase
• Ascorbate peroxidase
• Cucumber Cucumis sativus L
• Total chlorophyll content
• Anther culture
• Agar concentration
• Embryo maturation
• Cotyledonary embryos
• Gene action
• Parents and hybrids
• Quality traits in cucumber
• Gene action for yield
• Genetic diversity
• Tocher’s method
• Intra and inter cluster distance
• Yield parameters in cucumber
• PSB and Trichoderma viride
• Organic nutrient management
• Growth and flowering
• Investigation entitled
• Traits and phenotypic selection etc
• Factor causing genetic diversity
• Related and geographical
• Yield traits
• NAA and pusa uday
• Yield and yield traits
• Yield traits of cucumber
• Foliar application
• Plant growth regulators
• Testing of hybrid vigour
• Sex phenology in cucumber
• Hybrid vigour for sex phenology
• Desirable significant positive heterosis
• Poly house
• Growth and yield of cucumber
• Irrigation levels
• Yield of cucumber
• Xác định lượng nước tưới phù hợp
• Cây dưa leo
• Lượng nước tưới cho cây dưa leo
• Bốn loại giá thể
• Growth regulators
• Polyhouse condition
• Plant growth regulators on growth
• Quality parameters in cucumber
• Protein profiling
• SDS PAGE
• Monoecious cucumber (Cucumis sativus L )
• Seed protein profiles
• Genetic variability
• Additive gene
• Protected structure
• Genetic advance in cucumber
• Downy mildew
• Downy mildew in cucumber
• Fungicide mixtures against Pseudoperonospora cubensis
• Quantity of cucumber in countries
• Indirect selection
• Seed traits
• Fruit yield in cucumber
• Seed traits with fruit yield in cucumber
• Genetic divergence
• Horticultural traits in cucumber
• Divergence studies
• Exotic sources were planted
• Quantitative traits
• Cucumber (Cucumis sativus L ) germplams
• Genetic variability among 78 cucumber
• Cucumber germplasm collected