TAILIEUCHUNG - Genetic diversity and population structure of common bean (Phaseolus vulgaris L.) accessions through retrotransposon-based interprimer binding sites (iPBSs) markers
The common bean (Phaseolus vulgaris L.) is an important crop throughout the world and is highly recommended for many developing countries due to its nutritional components. A retrotransposon-based DNA marker system, interprimer binding sites (iPBSs), developed from reverse transcriptase primer binding sites, was used to determine genetic diversity in common bean accessions. | Turkish Journal of Agriculture and Forestry Turk J Agric For (2015) 39: 940-948 © TÜBİTAK doi: Research Article Genetic diversity and population structure of common bean (Phaseolus vulgaris L.) accessions through retrotransposon-based interprimer binding sites (iPBSs) markers 1 2 2, Seda NEMLİ , Tala KIANOOSH , Muhammed Bahattin TANYOLAÇ * Department of Genetics and Bioengineering, Faculty of Engineering and Natural Sciences, Gümüşhane University, Gümüşhane, Turkey 2 Department of Bioengineering, Faculty of Engineering, Ege University, Bornova, İzmir, Turkey 1 Received: Accepted/Published Online: Printed: Abstract: The common bean (Phaseolus vulgaris L.) is an important crop throughout the world and is highly recommended for many developing countries due to its nutritional components. A retrotransposon-based DNA marker system, interprimer binding sites (iPBSs), developed from reverse transcriptase primer binding sites, was used to determine genetic diversity in common bean accessions. The genetic polymorphism and effectiveness of the marker system were evaluated. A total of 180 polymorphic bands were detected using 47 iPBS primers among 67 common bean accessions, with an average of 4 polymorphic fragments per primer. The genetic similarity between accessions was calculated using the software NTSYS-pc and ranged from to . The average polymorphism information content value for the iPBS markers was . A model-based clustering method classified the common bean accessions into 4 populations using STRUCTURE software. The results indicated that the iPBS marker method can successfully determine the genetic diversity level in common bean accessions. Key words: Common bean, retrotransposon, iPBS, population structure, genetic diversity 1. Introduction The common bean (Phaseolus vulgaris L.) is one of the most ancient legumes and one of the five cultivated
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