TAILIEUCHUNG - Evaluation of antiproliferative and protective effects of Eupatorium cannabinum L. extracts
Eupatorium cannabinum L. (Asteraceae) has been used for a long time for medicinal purposes due to its various pharmacological effects and richness in active compounds such as phenolics, sesquiterpenes, pyrrolizidine alkaloids, and polysaccharides. | Turkish Journal of Biology Turk J Biol (2018) 42: 341-351 © TÜBİTAK doi: Research Article Evaluation of antiproliferative and protective effects of Eupatorium cannabinum L. extracts 1, 2 3 2 Alice GRIGORE *, Georgeta NEAGU , Nicoleta DOBRE , Adrian ALBULESCU , 4 4 2 Lucian IONITA , Carmen IONITA , Radu ALBULESCU 1 Department of Pharmaceutical Biotechnologies, National Institute of Chemical-Pharmaceutical Research and Development (ICCF), Bucharest, Romania 2 Department of Pharmacology, National Institute of Chemical-Pharmaceutical Research and Development (ICCF), Bucharest, Romania 3 Department of Analytics, National Institute of Chemical-Pharmaceutical Research and Development (ICCF), Bucharest, Romania 4 Department of Internal Medicine, Faculty of Veterinary Medicine, University of Agronomic Sciences and Veterinary Medicine of Bucharest, Bucharest, Romania Received: Accepted/Published Online: Final Version: Abstract: Eupatorium cannabinum L. (Asteraceae) has been used for a long time for medicinal purposes due to its various pharmacological effects and richness in active compounds such as phenolics, sesquiterpenes, pyrrolizidine alkaloids, and polysaccharides. Despite the high content of compounds that have important roles in medicinal plants, there are still limited literature data regarding this valuable species. The plant was fractioned using chloroform (EC) and distilled water (EA) and HPLC analysis revealed the presence of eupatorin, eupatilin, and quercetin in EC and caffeic acid and rutin in EA. The antiproliferative potential on BT-20, HepG2, Caco-2, and Jurkat cancer cell lines was assessed by MTS test. Jurkat cells were more sensitive to both extracts (IC50 of ± for EC and ± µg/mL for EA), while the other lines were susceptible only to EC (IC50 ± on Caco-2 cells and over 100 µg/mL on BT20 and HepG2 cells) after 24 h
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