TAILIEUCHUNG - Effects of gold nanorods modified with antiepidermal growth factor receptor monoclonal antibody on laryngeal cancer cells
We aimed to evaluate the photothermal effects of gold nanorods (GNRs) modified with antiepidermal growth factor receptor (EGFR) monoclonal antibody (mAb) on laryngeal cancer cells. EGFR protein expressions in HEP-2 cells, and normal laryngeal epithelial and laryngeal cancer tissues were detected by western blot. | Turkish Journal of Biology Turk J Biol (2018) 42: 144-151 © TÜBİTAK doi: Research Article Effects of gold nanorods modified with antiepidermal growth factor receptor monoclonal antibody on laryngeal cancer cells 1, 2, 1 1 1 3 4, 1 Fangzhou LIU †, Yan ZHU †, Yichuan QIAN , Yanbin ZHAO , Xiaotong ZHAO , Jia ZHANG , Yu ZHANG *, Yuan ZHANG 1 Department of Head and Neck Surgery, Jiangsu Cancer Hospital Affiliated to Nanjing Medical University, Nanjing, P. R. China 2 Department of Pathology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, P. R. China 3 PET-CT Department, Jiangsu Cancer Hospital Affiliated to Nanjing Medical University, Nanjing, P. R. China 4 School of Biological Sciences and Medical Engineering, Southeast University, Nanjing, P. R. China Received: Accepted/Published Online: Final Version: Abstract: We aimed to evaluate the photothermal effects of gold nanorods (GNRs) modified with antiepidermal growth factor receptor (EGFR) monoclonal antibody (mAb) on laryngeal cancer cells. EGFR protein expressions in HEP-2 cells, and normal laryngeal epithelial and laryngeal cancer tissues were detected by western blot. The cytotoxicity against HEP-2 and BEAS-2B cells was tested by MTT assay, and the photothermal effects were assessed by near-infrared (NIR) irradiation. The apoptosis of HEP-2 cells was detected by flow cytometry. EGFR expression in laryngeal cancer tissues was significantly higher than that in normal tissues (P ) at the irradiation power of ≤ w/ cm2. The survival rates of the GNRs and anti-EGFR mAb/ GNRs groups were significantly lower than that of the irradiation group at and w/cm2 (P ). . Cell apoptosis after NIR irradiation detected by flow cytometry Flow cytometry exhibited that the apoptosis rate of the anti-EGFR mAb/GNRs group was significantly higher than that of the GNRs group, and the .
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