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We aimed to evaluate the photothermal effects of gold nanorods (GNRs) modified with antiepidermal growth factor receptor (EGFR) monoclonal antibody (mAb) on laryngeal cancer cells. EGFR protein expressions in HEP-2 cells, and normal laryngeal epithelial and laryngeal cancer tissues were detected by western blot. | Turkish Journal of Biology Turk J Biol (2018) 42: 144-151 © TÜBİTAK doi:10.3906/biy-1708-101 http://journals.tubitak.gov.tr/biology/ Research Article Effects of gold nanorods modified with antiepidermal growth factor receptor monoclonal antibody on laryngeal cancer cells 1, 2, 1 1 1 3 4, 1 Fangzhou LIU †, Yan ZHU †, Yichuan QIAN , Yanbin ZHAO , Xiaotong ZHAO , Jia ZHANG , Yu ZHANG *, Yuan ZHANG 1 Department of Head and Neck Surgery, Jiangsu Cancer Hospital Affiliated to Nanjing Medical University, Nanjing, P. R. China 2 Department of Pathology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, P. R. China 3 PET-CT Department, Jiangsu Cancer Hospital Affiliated to Nanjing Medical University, Nanjing, P. R. China 4 School of Biological Sciences and Medical Engineering, Southeast University, Nanjing, P. R. China Received: 31.08.2017 Accepted/Published Online: 14.11.2017 Final Version: 27.04.2018 Abstract: We aimed to evaluate the photothermal effects of gold nanorods (GNRs) modified with antiepidermal growth factor receptor (EGFR) monoclonal antibody (mAb) on laryngeal cancer cells. EGFR protein expressions in HEP-2 cells, and normal laryngeal epithelial and laryngeal cancer tissues were detected by western blot. The cytotoxicity against HEP-2 and BEAS-2B cells was tested by MTT assay, and the photothermal effects were assessed by near-infrared (NIR) irradiation. The apoptosis of HEP-2 cells was detected by flow cytometry. EGFR expression in laryngeal cancer tissues was significantly higher than that in normal tissues (P 0.05) at the irradiation power of ≤2.4 w/ cm2. The survival rates of the GNRs and anti-EGFR mAb/ GNRs groups were significantly lower than that of the irradiation group at 3.2 and 4.0 w/cm2 (P 0.05). 3.4. Cell apoptosis after NIR irradiation detected by flow cytometry Flow cytometry exhibited that the apoptosis rate of the anti-EGFR mAb/GNRs group was significantly higher than that of the GNRs group, and the .
