TAILIEUCHUNG - Comparative proteomic analysis of Bacillus thuringiensis wild-type and two mutant strains disturbed in polyphosphate homeostasis

Polyphosphate polymer (polyP) plays a very important role in every living cell. Synthesis of this linear polymer of phosphate (Pi) residues is catalyzed by the polyphosphate kinase (PPK) enzyme. | Turkish Journal of Biology Turk J Biol (2018) 42: 87-102 © TÜBİTAK doi: Research Article Comparative proteomic analysis of Bacillus thuringiensis wild-type and two mutant strains disturbed in polyphosphate homeostasis 1 2 2 2, Filiz YEŞİLIRMAK , Tuğrul DORUK , Şerif YILMAZ , Sedef TUNCA GEDİK * Department of Chemistry, Faculty of Science, İzmir Institute of Technology, Urla, İzmir, Turkey 1 Department of Molecular Biology and Genetics, Faculty of Sciences, Gebze Technical University, Gebze, Kocaeli, Turkey 1 Received: Accepted/Published Online: Final Version: Abstract: Polyphosphate polymer (polyP) plays a very important role in every living cell. Synthesis of this linear polymer of phosphate (Pi) residues is catalyzed by the polyphosphate kinase (PPK) enzyme. It was shown that high levels of intracellular polyphosphate stimulated endotoxin production by Bacillus thuringiensis subsp. israelensis (Bti). In this study, proteomic analysis of the wild-type and two mutant strains, overexpressing the ppk gene (Bti pHTppk) and without the ppk gene (Bti ∆ppk), were used to clarify the relation between polyP and endotoxin production. Intracellular proteins were separated by two-dimensional gel electrophoresis; 41 spots of interest (proteins differentially expressed) were obtained and 35 of them were identified by mass spectrometry. Analysis of the protein profiles showed that there is a general decrease in the expression levels of proteins related with energy metabolism, amino acid metabolism, and purine biosynthesis in both Bti pHTppk and Bti ∆ppk. Gluconeogenesis and fatty acid metabolism were also slowed down in both strains, whereas expression of stress response proteins increased compared to the wild-type. These results suggested that changes in polyP concentration cause a general stress condition inside the cell, which in turn stimulates secondary metabolite .

• Sáng kiến kinh nghiệm
• Polyphosphate polymer
• Polyphosphate kinase
• Two dimensional gel electrophoresis
• Bacillus thuringiensis israelensis
• Secondary metabolite
• Ammonium polyphosphate
• Boron phosphate
• Flame retardants
• Wood polymer composite
• Boric acid
• Boron compounds
• BMC Chemistry
• Mechanical properties
• Flame retardancy
• Incorporating polypropylene
• Polymer materials
• Isolation and evaluation
• Secondary metabolite producing
• Tetranychus urticae on okra
• Spider mite
• Rhizobium leguminosarum sp LNRLS9
• Antimicrobial activity
• Root nodules
• Secondary metabolite profiling
• Unravelling the antibiotic susceptibility
• MS medium
• Callus induction
• In vitro production of secondary metabolite
• Andrographis echioides (L ) nees
• In vitro production
• Turkish Journal of Agriculture and Forestry
• Tạp chí Nông Lâm nghiệp
• Plant tissue culture
• Plant secondary metabolite production
• Medicinal and aromatic plants
• Strong phyto constituents
• Xanthomonas axanopodis pv alii
• Secondary metabolite production
• Trichoderma spp
• Potential as antibacteria
• Trichoderma filtrate
• Streptomyces sp
• Molecular docking studies
• Bacterial leaf blight pathogen of rice
• Bacterial leaf blight pathogen
• Novel secondary metabolite
• BMC Genomics
• Fusarium incarnatum equiseti species complex
• Comparative genome analyses
• Secondary metabolite genes
• Horizontal gene transfer
• Cordyceps militaris
• Entomopathogenic fungi
• SMRT sequencing
• Adventitious roots
• Antioxidant activity
• Codonopsis javanica
• Light condition
• Aspergillus japonicus
• HeLa cells
• Include biologically
• Biochemical pathways
• DNA fragmentation analysis
• Caspase 3 enzyme activity assays
• BMC Plant Biology
• Fruit quality
• Liquid chromatography
• Mass spectrometry
• Secondary metabolites
• Gene loss
• Biosynthetic gene cluster
• Genomic comparison
• Botcinic acid
• Metabolite repertoire
• Stevia rebaudiana
• Crop ecology
• Plant nutrition
• Spatial variability
• Endophytic fungus
• Calcarisporium arbuscula
• RNA Seq assays
• Backbone biosynthesis
• Genome mining
• Comparative genomics
• Gene cluster family
• Secondary metabolite diversity
• Journal of Botany
• Intracellular secretory structures
• Medicinal plant
• Metabolites resources
• Histochemical based techniques
• trình bày báo cáo
• tài liệu báo cáo khoa học
• nghiên cứu y học
• báo cáo y học
• kiến thức y học
• Catharanthus roseus
• Photosynthetic pigment
• Salt affected soil
• NaCl treated plants
• Alternative therapeutic approaches
• Symbiotic organisms of fungi
• Usnic acid
• Significant biological activities
• Nonmalignant cell lines
• AsA GSH cycle
• Oxidative damage
• Triticum aestivum
• Abiotic stress factor
• Scavenging activity
• Metabolite profiling
• Fatty acids
• Temporal biosynthesis pathway
• Dynamic parent of origin
• Maize endosperm
• Days after pollination
• Secondary metabolite biosynthetic process
• Kandelia obovata
• Sonneratia caseolaris
• Total phenolic
• Elemental ion contents of leaves
• Caseolaris forests located
• Bacillus subtilis
• Metabolite biosynthetic gene
• Monascus species
• Polyketide synthase
• Natural pigment
• Food microbiology