TAILIEUCHUNG - Clinical effects of embryos culture in low oxygen concentration

Objectives: To compare pregnancy rate, live birth rate between 2 groups 5% O2 and 20% O2. Subjects and method: This is a randomized clinical trial study among 172 patients who were divided into 2 groups: embryos cultured in 5% and 20% oxygen concentrationture in low oxygen concentration. | Journal of military pharmaco-medicine n08-2017 CLINICAL EFFECTS OF EMBRYOS CULTURE IN LOW OXYGEN CONCENTRATION Nguyen Thi Minh*; Nguyen Viet Tien*; Quan Hoang Lam** SUMMARY Objectives: To compare pregnancy rate, live birth rate between 2 groups 5% O 2 and 20% O2. Subjects and method: This is a randomized clinical trial study among 172 patients who were divided into 2 groups: embryos cultured in 5% and 20% oxygen concentration. Before culture, using FSH to stimulate ovaries. Oocytes retrieval after hCG injection 36 hours. Fertilization by conventional IVF or ICSI. On day 3, 3 - 5 top quality embryos were selected to culture to day 5 in both group. Residual embryos were frozen by vitrification method. Results: There were no differences of characteristics, of ovarian reserve, ovarian stimulation and embryos transfer in both groups. Clinical pregnancy rate in 5% oxygen group was of , significantly higher than 40,69% in 20% oxygen group. Live birth rate in 5% oxygen group was () higher than () in 20% oxygen group but no significantly. Conclusion: Clinical pregnancy rate on day 5 in 5% oxygen group was higher than that in 20% oxygen group. Live birth rate in 5% oxygen group was higher than in 20% oxygen group but no significantly. * Keywords: In vitro fertilization; Pregnancy rate; Live birth rate: Low oxygen concentration. INTRODUCTION In the field of in vitro fertilization (IVF), techniques increasingly approach more physiological conditions in order to improve the success rate. Many studies in the world state that blastocyst transfer is closely related to physiology and selects embryos of good quality andhigh viability, helping increase the rate of implantation, pregnancy, and rate of live births. Moreover, many experimental studies showed that the concentration of oxygen is only about 5 - 8% in the heart of tubal, and only . 3. Embryo transfer results. Table 3: Embryo transfer and pregnancy results. Characteristics Oxygen group 5% Oxygen .

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