TAILIEUCHUNG - The role of protein synthesis on the adhesion strength of CHL cells

Cells require protein synthesis to perform the adhesion function properly. In order to discover the effect of endogenous protein synthesis on CHL cell adhesion, the adhesion strengths of cultured Chinese Hamster Lung (CHL) cells that were grown for 24 hours on the glass surface in the presence of 1µgml-1 of cycloheximide or emetine or, both were measured quantitatively using a converging laminar flow chamber. | Tr. J. of Biology 22 (1998) 397-405 © TÜBİTAK The Role of Protein Synthesis on the Adhesion Strength of CHL Cells Ali YILDIRIM Atatürk University, K. Karabekir Education Faculty, Department of Chemistry, Erzurum-TURKEY William JD WHİSH Department of Biochemistry, University of Bath, Bath BA2 7AY UK Received: Abstract: Cells require protein synthesis to perform the adhesion function properly. In order to discover the effect of endogenous protein synthesis on CHL cell adhesion; the adhesion strengths of cultured Chinese Hamster Lung (CHL) cells that were grown for 24 hours on the glass surface in the presence of 1µgml-1 of cycloheximide or emetine or, both were measured quantitatively using a converging laminar flow chamber. The inhibition of protein synthesis by above drug(s) reduced cell adhesion strength significantly. Hence critical shear stress of detachment of cell were ± Nm-2 , ± , and ± , respectively. While that of the control cells was ± . Before subculturing CHL cells in the presence of these drug(s), pre-treatment of cells with the same protein synthesis inhibitor even further reduced cell adhesion strength. Thus with pre-treatment of cycloheximide, emetine, both emetine and cycloheximide critical shear stress of detachment of cells were ± , ± , and ± , respectively. These results indicate that cells requires protein synthesis to gain possible maximum adhesion strength. Key Words: CHL cell, cell adhesion, protein synthesis, emetine, cycloheximide. CHL Hücrelerinin Yapışma Kuvvetinde Protein Sentezinin Rolü Özet: Hücreler yapışma fonksiyonlarını tam olarak gerçekleştirebilmeleri için protein sentezine ihtiyaç duyarlar. Protein sentezinin hücre yapışmasındaki etkisini incelemek için; cam yüzeyinde 24 saat süre ile 1µ g/ml emetine, cycloheximide veya ikisininde mevcudiyetinde büyüyen kültür Chinese Hamster Lung (CHL) hücrelerinin yapışma kuvveti düzgün ve giderek

• Sinh học
• CHL cell
• Cell adhesion
• Protein synthesis
• Extracellular matrix
• Neubave hemocytometer