TAILIEUCHUNG - Purification of antioxidant protein isolated from Peganum harmala and its protective effect against CCl4 toxicity in rats

The present study was conducted to determine the protective effect of the purified protein from seeds of Peganum harmala against carbon tetrachloride (CCl4)-induced toxicity in male albino rats. | Turkish Journal of Biology Turk J Biol (2013) 37: 39-48 © TÜBİTAK doi: Research Article Purification of antioxidant protein isolated from Peganum harmala and its protective effect against CCl4 toxicity in rats 1, 2 2 Hanaa AHMED *, Helal ABU EL ZAHAB , Gamia ALSWIAI Department of Botany, Faculty of Science, Cairo University, 12613 Giza, Egypt 2 Department of Zoology, Faculty of Science, Cairo University, 12613 Giza, Egypt 1 Received: Accepted: Published Online: Printed: Abstract: The present study was conducted to determine the protective effect of the purified protein from seeds of Peganum harmala against carbon tetrachloride (CCl4)-induced toxicity in male albino rats. The purification steps included ammonium sulfate fractionation and chromatography on DEAE-cellulose, CM-Sepharose, and Superdex 75 columns. The molecular mass of the purified protein was 132 kDa by gel filtration technique; it consisted of 2 subunits with molecular masses of kDa and kDa by SDS-PAGE. Results of the dose-dependent experiment with purified protein prior to CCl4 administration were higher at 4 mg/kg body weight. The antioxidant activity of the purified protein was determined in vitro by DPPH radical scavenging test. Administration of CCl4 significantly increased the activities of alanine aminotransferase, aspartate aminotransferase, and alkaline phosphatase in serum. However, a significant decrease in the level of total serum protein as well as the activities of superoxide dismutase, catalase, and reduced glutathione in liver tissues, and a significant increase in malondialdehyde level, were recorded. Pretreatment with 4 mg/kg body weight of the purified protein significantly altered the deteriorating damage induced by CCl4 toxicity to a near normal range, which was similar to treatment with vitamin C. These results suggest that the purified protein possesses

• Sinh học
• Peganum harmala
• Hepatic toxicities
• Alanine aminotransferase
• Aspartate aminotransferase
• Alkaline phosphatase
• Superoxide dismutase
• C value
• Flow cytometry
• Iberian Peninsula
• Nuclear DNA amount
• Somatic chromosome number
• Pattern analysis
• Redundancy analysis
• Lower Chelif
• Lear clumped pattern
• Geographic information systems
• Acacia cyanophylla Lindl
• Weeds Peganum harmala L
• Silybum marianum L
• Petroleum ether
• Ethyl acetate
• Gypsum desert
• Salsola arbuscula pall
• Degraded pastures
• Peganum harmala and agrobiocenosis
• Carnabchul gypsum desert
• scientific reports
• thesis report
• medical report
• Much of the toxicity of Hb has been linked to its redox activity; Hb may generate reactive oxygen species
• such as the superoxide anion
• Superoxide is intrinsically toxic
• and superoxide dismutase (SOD) provides important cellular protection
• However
• if the Hb molecule is located outside the red blood cell
• the normal protection systems involving SOD and catalase are no longer closely associated with it
• exposing Hb and its cellular surround
• Sắc ký trao đổi ion
• Superoxide dismutase
• Tôm sú (Penaeus monodon)
• Tính chất của Superoxide Dismuatase
• Tinh sạch một phần Superoxide dismutase
• breast cancer
• medicine
• Aeropyrum
• chemical reaction
• Oxidative stress
• Manganese superoxide dismutase
• Glutathione peroxidase
• Reactive oxygen species
• Mitochondrial oxidative metabolism
• Sorghum bicolor
• Genome wide analysis
• In silico analysis
• Hydrogen peroxide
• Hydroxyl radical
• Antioxidant system
• Lipid peroxidation
• Iron superoxide dismutase
• Ascorbate dependent peroxidase
• Total carotenoid
• BMC Plant Biology
• Manihot esculenta Crantz
• Cytosolic superoxide dismutase
• Cytosolic ascorbate peroxidase
• Reactive oxygen species scavenging
• Abiotic stress resistance
• Pangasianodon hypophthlmus
• Effects of thermal stress
• Dietary zinc on growth performance
• Superoxide dismutase–1
• Catalase enzyme activity
• Pangasianodon hypophthalmus
• BMC Pulmonary Medicine
• Lung function
• Nitric oxide
• Positive pressure mechanical ventilation
• Surfactant protein A
• BMC Genomics
• Gene family
• Brassica juncea
• B rapa
• Abiotic stress
• Differential expression
• Oxy cao áp
• Hoạt độ Enzym superoxide dismutase
• Nhồi máu não
• Điều trị oxy cao áp
• Y học cao áp lâm sàng
• Y học dưới nước
• Ascorbate peroxidase
• Stress condition
• Tomato (Solanum lycopersicum Mill)
• Superoxide dismutasegenes alteration
• Tạp chí Y học Quân sự
• Chronic gastritis
• H
• pylori
• Alternative splicing
• Antioxidant enzymes
• BMC Biotechnology
• Heterologous expression
• CD spectroscopy
• Homology modelling
• Peroxidase activity
• trình bày báo cáo
• tài liệu báo cáo khoa học
• nghiên cứu y học
• báo cáo y học
• kiến thức y học
• Durum wheat
• Superoxide dismutases
• Oxygen inactivate enzymes
• Chlorophyll content index
• Journal of Chemistry
• Organic sulfur containing compounds
• Superoxide anion radical
• In silico
• Cyclic voltammetry
• scientific research
• biochemistry
• genetics
• Structural biochemistry
• Structural Biology
• tài liệu báo cáo nghiên cứu khoa học
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