TAILIEUCHUNG - Lecture Molecular biology (Fifth Edition): Chapter 24 - Robert F. Weaver

Chapter 24 introduction to genomics: DNA sequencing on a genomic scale. We will begin this chapter with a discussion of positional cloning, a technique for identifying a gene responsible for a given trait, and see how much easier this process is when the sequence of the organism’s genome is known. Then we will examine the techniques scientists use to sequence DNA on a massive scale. We will also discuss some of the lessons we have learned from the sequences of genomes, especially the evolutionary insights to be gained by comparing the genomic sequences of different organisms. | Molecular Biology Fifth Edition Chapter 24 Introduction to Genomics: DNA Sequencing on a Genomic Scale Lecture PowerPoint to accompany Robert F. Weaver Copyright © The McGraw-Hill Companies, Inc. Permission required for reproduction or display. Positional Cloning Positional cloning is a method for the discovery of genes involved in genetic traits Positional cloning was very difficult in the absence of genomic information Begins with mapping studies to pin down the location of the gene of interest to a relatively small region of DNA 24- Classical Tools of Positional Cloning Mapping depends on a set of landmarks to which gene position can be related Restriction Fragment Length Polymorphisms (RFLP) are landmarks with lengths of restriction fragments given by a specific enzyme that vary from one individual to another Exon Traps use a special vector to help clone exons only CpG Islands are DNA regions containing unmethylated CpG sequences 24- Detecting RFLPs 24- Exon .

• Sinh học
• Molecular biology
• Lecture Molecular biology
• Sinh học phân tử
• Bài giảng Sinh học phân tử
• Positional cloning
• Genomic sequencing
• High Yield cell and molecular biology
• Cell and molecular biology
• The cell cycle
• Molecular biology of cancer
• Cell biology of the immune system
• Molecular biology techniques
• Identification of human disease genes
• Campbell biology
• Lecture Campbell biology
• Biological sciences
• Systems biology
• Molecular biology of the gene
• Chromosome replication
• Meiosis and genetic recombination
• The human nuclear genome
• The human mitochondrial genome
• Molecular cloning methods
• Gene cloning
• Directional cloning
• Amino acids
• Molecular tools
• Studying genes
• History of genetics
• Both simple
• Molecular genetics
• Genetic code
• Molecular biology of microorganisms
• Different facets
• Plant molecular biology
• Genetic manipulation
• Recombinant DNA technology
• Molecular diagnostics
• Biochemistry cell and molecular biology test
• Molecular Biology Test
• Biochemistry Test
• Genetics test
• Structural Biology
• Cupramolecular complexes
• Lecture Biology
• Mendel’s Laws
• Transmission genetics
• Mendel’s gene transmission
• Genetic material
• Transforming material
• DNA structure
• Gene function
• Storing information
• Mechanism of transcription
• RNA polymerase structure
• Lac operon
• Bacterial transcription
• Sigma factor switching
• DNA Protein interactions
• The λ family of repressors
• Eukaryotic RNA polymerases
• RNA polymerases
• Class II factors
• Transcription activators in eukaryotes
• RNA polymerase II
• Histones in the nucleosome
• Nucleosome structure
• Messenger RNA processing I
• Genes in pieces
• RNA processing II
• Cap structure
• Cytoplasmic polyadenylation
• RNA processing
• Ribosomal RNA processing
• Transfer RNA processing